First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766)

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dc.contributor.authorSpera, Caroline Giuseppa
dc.contributor.authorLavorente, Fernanda Louise Pereira
dc.contributor.authorLorenzetti, Elis
dc.contributor.authorCalasans Marques, Gustavo de [UNESP]
dc.contributor.authorAlmeida Freitas, Luana de
dc.contributor.authorMartins, Maira Bonamin [UNESP]
dc.contributor.authorTeixeira, Carlos Roberto [UNESP]
dc.contributor.authorAlfieri, Amauri Alcindo
dc.contributor.authorAlfieri, Alice Fernandes
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniv Pitagoras Unopar
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T12:23:45Z
dc.date.available2021-06-25T12:23:45Z
dc.date.issued2020-10-23
dc.description.abstractThe crab-eating fox (Cerdocyon thous) is a small wild mammal present in all Brazilian biomes and in some countries of South America. This study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (Cerdocyon thous) in Brazil. The Center for Medicine and Research of Wild Animals of the Universidade Estadual Paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. After 13 days, the animal presented anorexia, diarrhea, fever, prostration, and neurological signs progressing to death with an inconclusive diagnosis. In a retrospective study, tissue fragments stored at - 80 degrees C were used to identify nucleic acids from major canine viruses, such as canine parvovirus-2 (CPV-2), canine adenovirus A types 1 and 2, canid alphaherpesvirus 1, and canine distemper virus. The amplified product with the expected length for CPV-2 was obtained from the heart fragment. After performing nucleotide (nt) sequencing of the amplicon, it was possible to demonstrate that the crab-eating fox strain exhibited high (99.8%) nt identity with the CPV-2b prototype (CPV-39 strain). Additionally, deduced amino acid (aa) sequence analysis showed the GAT codon for the aa Asp (D) at position 426 of the CPV-2 viral protein VP2, which characterizes the subtype 2b. To the best of the authors' knowledge, this report describes the first detection of CPV-2b DNA in tissue fragments from a crab-eating fox.en
dc.description.affiliationUniv Estadual Londrina, Lab Anim Virol, Dept Vet Prevent Med, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, Brazil
dc.description.affiliationUniv Estadual Londrina, Multiuser Anim Hlth Lab, Mol Biol Unit, Dept Vet Prevent Med, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, Brazil
dc.description.affiliationUniv Pitagoras Unopar, Post Grad Program Anim Hlth & Prod, Arapongas, Parana, Brazil
dc.description.affiliationUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Vet Hosp, Anim Pathol, POB 560, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Vet Hosp, Anim Pathol, POB 560, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipBrazilian institute: Financing of Studies and Projects (FINEP)
dc.description.sponsorshipBrazilian institute: Araucaria Foundation (FAP/PR)
dc.description.sponsorshipFunadesp
dc.format.extent449-453
dc.identifierhttp://dx.doi.org/10.1007/s42770-020-00389-9
dc.identifier.citationBrazilian Journal Of Microbiology. New York: Springer, v. 52, n. 1, p. 449-453, 2021.
dc.identifier.doi10.1007/s42770-020-00389-9
dc.identifier.issn1517-8382
dc.identifier.urihttp://hdl.handle.net/11449/209607
dc.identifier.wosWOS:000583488700001
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.sourceWeb of Science
dc.subjectCerdocyon thous
dc.subjectWild
dc.subjectHeart
dc.subjectCPV-2b
dc.titleFirst detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766)en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer

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