Purification and structural characterisation of (1 -> 3 ; 1 -> 6)-beta-D-glucans (botryosphaerans) from Botryosphaeria rhodina grown on sucrose and fructose as carbon sources: a comparative study

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dc.contributor.authorda Silva, MDC
dc.contributor.authorIzeli, N. L.
dc.contributor.authorMartinez, P. F.
dc.contributor.authorSilva, I. R.
dc.contributor.authorConstantino, CJL
dc.contributor.authorCardoso, M. S.
dc.contributor.authorBarbosa, A. M.
dc.contributor.authorDekker, RFH
dc.contributor.authorda Silva, GVJ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:22:53Z
dc.date.available2014-05-20T13:22:53Z
dc.date.issued2005-07-04
dc.description.abstractTwo botryosphaerans, exopolysaccharides (EPS) secreted by the ascomyceteous fungus Botryosphaeria rhodina, when grown on sucrose and fructose as sole carbon sources, were structurally compared after their isolation from the culture medium. Both EPS were submitted to trypsin digestion, and eluted as a single peak on gel filtration. Total acid hydrolysis yielded only glucose, and data from methylation analysis and Smith degradation indicated that both EPS constituted a main chain of glucopyranosyl beta(1 -> 3) linkages substituted at O-6. The products obtained after partial acid hydrolysis demonstrated side chains consisting of glucosyl- and gentiobiosyl- linked beta(1 -> 6) residues. C-13-NMR spectroscopy studies showed that all glucosidic linkages were of the beta-configuration. The carbon source affected the side chain structures of botryosphaeran but not the main chain makeup. Sucrose produced less branching (21%) than fructose (31%). (c) 2005 Published by Elsevier Ltd.en
dc.description.affiliationUniv Estadual Paulista, Dept Fis, Fac Ciências & Tecnol Quim & Biol, BR-19060900 Presidente Prudente, SP, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Bioquim & Biotecnol, CCE, BR-86051990 Londrina, PR, Brazil
dc.description.affiliationUniv São Paulo, Dept Quim, Fac Filosofia Ciências & Letras Ribeirao Pret, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Fis, Fac Ciências & Tecnol Quim & Biol, BR-19060900 Presidente Prudente, SP, Brazil
dc.format.extent10-17
dc.identifierhttp://dx.doi.org/10.1016/j.carbpol.2005.01.002
dc.identifier.citationCarbohydrate Polymers. Oxford: Elsevier B.V., v. 61, n. 1, p. 10-17, 2005.
dc.identifier.doi10.1016/j.carbpol.2005.01.002
dc.identifier.issn0144-8617
dc.identifier.urihttp://hdl.handle.net/11449/6788
dc.identifier.wosWOS:000230220800003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofCarbohydrate Polymers
dc.relation.ispartofjcr5.158
dc.relation.ispartofsjr1,428
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBotryosphaeria rhodinapt
dc.subjectexopolysaccharidespt
dc.subjectbeta(1 -> 3; 1 -> 6)-D-glucanspt
dc.subjectcarbon sourcept
dc.subjectstructural variationpt
dc.titlePurification and structural characterisation of (1 -> 3 ; 1 -> 6)-beta-D-glucans (botryosphaerans) from Botryosphaeria rhodina grown on sucrose and fructose as carbon sources: a comparative studyen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-7732-2679[1]
unesp.author.orcid0000-0002-2339-8985[7]
unesp.author.orcid0000-0003-0604-5536[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept

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Artigos - Física, Química e Biologia - FCT