Short communication: Acute but transient increase in serum insulin reduces messenger RNA expression of hepatic enzymes associated with progesterone catabolism in dairy cows

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dc.contributor.authorVieira, F. V R [UNESP]
dc.contributor.authorCooke, Reinaldo Fernandes [UNESP]
dc.contributor.authorAboin, A. C. [UNESP]
dc.contributor.authorLima, P. [UNESP]
dc.contributor.authorVasconcelos, José Luiz Moraes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEastern Oregon Agricultural Research Center
dc.date.accessioned2014-05-27T11:28:21Z
dc.date.available2014-05-27T11:28:21Z
dc.date.issued2013-02-01
dc.description.abstractThe objective of this experiment was to evaluate the effects of glucose infusion on serum concentrations of glucose, insulin, and progesterone (P4), as well as mRNA expression of hepatic CYP2C19 and CYP3A4 in nonlactating, ovariectomized cows in adequate nutritional status. Eight Gir × Holstein cows were maintained on a low-quality Brachiaria brizantha pasture with reduced forage availability, but they individually received, on average, 3. kg/cow daily (as fed) of a corn-based concentrate from d -28 to 0 of the experiment. All cows had an intravaginal P4-releasing device inserted on d -14, which remained in cows until the end of the experiment (d 1). On d 0, cows were randomly assigned to receive, in a crossover design containing 2 periods of 24. h each (d 0 and 1), (1) an intravenous glucose infusion (GLUC; 0.5. g of glucose/kg of BW, over a 3-h period) or (2) an intravenous saline infusion (SAL; 0.9%, over a 3-h period). Cows were fasted for 12. h before infusions, and they remained fasted during infusion and sample collections. Blood samples were collected at 0, 3, and 6. h relative to the beginning of infusions. Liver biopsies were performed concurrently with blood collections at 0 and 3. h. After the last blood collection of period 1, cows received concentrate and returned to pasture. Cows gained BW (16.5 ± 3.6. kg) and BCS (0.08 ± 0.06) from d -28 to 0. Cows receiving GLUC had greater serum glucose and insulin concentrations at 3. h compared with SAL cohorts. No treatment effects were detected for serum P4 concentrations, although mRNA expression of CYP2C19 and CYP3A4 after the infusion period was reduced for cows in the GLUC treatment compared with their cohorts in the SAL treatment. In conclusion, hepatic CYP3A4 and CYP2C19 mRNA expression can be promptly modulated by glucose infusion followed by acute increases in circulating insulin, which provides novel insight into the physiological mechanisms associating nutrition and reproductive function in dairy cows. © 2013 American Dairy Science Association.en
dc.description.affiliationDepartamento de Produção Animal Faculdade de Medicina Veterinária e Zootecnia UNESP, Botucatu, São Paulo 18618-000
dc.description.affiliationOregon State University Eastern Oregon Agricultural Research Center, Burns 97720
dc.description.affiliationDepartamento de Fisiologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu, São Paulo 18618-000
dc.description.affiliationUnespDepartamento de Produção Animal Faculdade de Medicina Veterinária e Zootecnia UNESP, Botucatu, São Paulo 18618-000
dc.description.affiliationUnespDepartamento de Fisiologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu, São Paulo 18618-000
dc.format.extent1085-1089
dc.identifierhttp://dx.doi.org/10.3168/jds.2012-5783
dc.identifier.citationJournal of Dairy Science, v. 96, n. 2, p. 1085-1089, 2013.
dc.identifier.doi10.3168/jds.2012-5783
dc.identifier.issn0022-0302
dc.identifier.issn1525-3198
dc.identifier.scopus2-s2.0-84872688308
dc.identifier.urihttp://hdl.handle.net/11449/74543
dc.identifier.wosWOS:000313678300038
dc.language.isoeng
dc.relation.ispartofJournal of Dairy Science
dc.relation.ispartofjcr2.749
dc.relation.ispartofsjr1,350
dc.relation.ispartofsjr1,350
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDairy cow
dc.subjectGlucose infusion
dc.subjectHepatic enzyme
dc.subjectProgesterone
dc.subjectcytochrome P450 3A
dc.subjectglucose
dc.subjectinsulin
dc.subjectmessenger RNA
dc.subjectprogesterone
dc.subjectunspecific monooxygenase
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectblood
dc.subjectcattle
dc.subjectenzymology
dc.subjectfemale
dc.subjectliver
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectAnimals
dc.subjectAryl Hydrocarbon Hydroxylases
dc.subjectCattle
dc.subjectCytochrome P-450 CYP3A
dc.subjectFemale
dc.subjectGlucose
dc.subjectInsulin
dc.subjectLiver
dc.subjectMetabolism
dc.subjectRNA, Messenger
dc.subjectBos
dc.subjectBrachiaria
dc.subjectUrochloa brizantha
dc.subjectZea mays
dc.titleShort communication: Acute but transient increase in serum insulin reduces messenger RNA expression of hepatic enzymes associated with progesterone catabolism in dairy cowsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.orcid0000-0001-6324-3614[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Produção Animal - FMVZ
Artigos - Fisiologia - IBB