Lack of effect of prior treatment with fluoride on genotoxicity of two chemical agents in vitro

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dc.contributor.authorRibeiro, D. A.
dc.contributor.authorMarques, Mariângela Esther Alencar [UNESP]
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-20T13:36:59Z
dc.date.available2014-05-20T13:36:59Z
dc.date.issued2007-01-01
dc.description.abstractThe goal of this study was to investigate the ability of fluoride to modulate the genotoxic effects induced by the oxidative agent hydrogen peroxide (H2O2) and the alkylating agent methyl methanesulfonate (MMS) in vitro by the single-cell gel ( comet) assay. Chinese hamster ovary cells were exposed in culture for 1 h at 37 degrees C to sodium fluoride at 7-100 mu g/ml. NaF-treated and control cells were then incubated with 0-10 mu M MMS in phosphate-buffered saline (PBS) for 15 min at 37 degrees C, or 7-100 mu M H2O2 in distilled water for 5 min on ice. Negative control cells were treated with PBS for 1 h at 37 degrees C. Clear concentration-related effects were observed for the two genotoxins. Increase of DNA damage induced by either MMS or H2O2 was not significantly altered by pretreatment with NaF. The data indicate that NaF does not modulate alkylation-induced genotoxicity or oxidative DNA damage as measured by the single-cell gel ( comet) assay. Copyright (c) 2007 S. Karger AG, Baselen
dc.description.affiliationUNESP, Fac Med Botucatu, TOXICAN Dept Patol, Botucatu Med Sch, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Hlth Sci, Santos, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med Botucatu, TOXICAN Dept Patol, Botucatu Med Sch, BR-18618000 Botucatu, SP, Brazil
dc.format.extent239-243
dc.identifierhttp://dx.doi.org/10.1159/000099326
dc.identifier.citationCaries Research. Basel: Karger, v. 41, n. 3, p. 239-243, 2007.
dc.identifier.doi10.1159/000099326
dc.identifier.issn0008-6568
dc.identifier.lattes5051118752980903
dc.identifier.lattes7528116925519142
dc.identifier.urihttp://hdl.handle.net/11449/12756
dc.identifier.wosWOS:000245758200014
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofCaries Research
dc.relation.ispartofjcr2.188
dc.relation.ispartofsjr1,108
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectgenotoxicitypt
dc.subjectsingle cell gel (comet) assaypt
dc.subjectsodium fluoridept
dc.titleLack of effect of prior treatment with fluoride on genotoxicity of two chemical agents in vitroen
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderKarger
unesp.author.lattes5051118752980903
unesp.author.lattes7528116925519142
unesp.author.orcid0000-0001-9323-3134[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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