Pharmacological treatment with galectin-1 protects against renal ischaemia-reperfusion injury

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dc.contributor.authorCarlos, Carla P. [UNESP]
dc.contributor.authorSilva, Analice A. [UNESP]
dc.contributor.authorGil, Cristiane D.
dc.contributor.authorOliani, Sonia M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFACERES School of Medicine Brazil
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:21:01Z
dc.date.available2018-12-11T17:21:01Z
dc.date.issued2018-12-01
dc.description.abstractGalectin-1 protein (GAL-1) has important anti-inflammatory properties, but related pharmacologic approaches to effectively treat or prevent renal ischaemia and reperfusion injury are highly limited. Here, we investigated the effect of GAL-1 in a renal ischaemia-reperfusion injury rat model and an in vitro hypoxia-reoxygenation model with a proximal renal tubular epithelial cell line. In vivo, pretreatment with GAL-1 attenuated the renal parameters changed by ischaemia-reperfusion/hypoxia-reoxygenation, with recovery of renal function, protecting against influx of leukocytes, cell death and oxidative stress. Ischaemia-reperfusion/hypoxia-reoxygenation was also associated with increased renal endogenous expression of GAL-1 and intercellular adhesion molecule 1 (ICAM-1) plus augmented levels of proinflammatory cytokines IL-1β, TNF-α and MCP-1 and decreased anti-inflammatory IL-10 in urine, all of which were abrogated by GAL-1 treatment. In vitro studies demonstrated renal tubular epithelial cells as an important source of GAL-1 during hypoxia-reoxygenation and confirmed the protective effects of exogenous GAL-1 through downregulation of proinflammatory cytokine release by proximal renal tubular epithelial cells. Collectively, our findings confirm the important anti-inflammatory role of GAL-1 in kidney ischaemia and reperfusion injury and indicate its promising use as a therapeutic approach.en
dc.description.affiliationDepartment of Biology Instituto de Biociências Letras e Ciências Exatas Sao Paulo State University UNESP
dc.description.affiliationDepartment of Medicine FACERES School of Medicine Brazil
dc.description.affiliationDepartment of Morphology and Genetics Federal University of Sao Paulo UNIFESP
dc.description.affiliationUnespDepartment of Biology Instituto de Biociências Letras e Ciências Exatas Sao Paulo State University UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2011/22113-6
dc.description.sponsorshipIdFAPESP: 2015/09858-3
dc.description.sponsorshipIdFAPESP: 2016/02012-4
dc.description.sponsorshipIdCNPq: 308144/2014-7
dc.identifierhttp://dx.doi.org/10.1038/s41598-018-27907-y
dc.identifier.citationScientific Reports, v. 8, n. 1, 2018.
dc.identifier.doi10.1038/s41598-018-27907-y
dc.identifier.file2-s2.0-85048964498.pdf
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-85048964498
dc.identifier.urihttp://hdl.handle.net/11449/176493
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.relation.ispartofsjr1,533
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titlePharmacological treatment with galectin-1 protects against renal ischaemia-reperfusion injuryen
dc.typeArtigo
unesp.author.orcid0000-0003-0516-4533[1]

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Artigos - Biologia - IBILCE