Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm

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dc.contributor.authorTrigo-Gutierrez, Jeffersson Krishan [UNESP]
dc.contributor.authorSanitá, Paula Volpato [UNESP]
dc.contributor.authorTedesco, Antonio Claudio
dc.contributor.authorPavarina, Ana Cláudia [UNESP]
dc.contributor.authorMima, Ewerton Garcia de Oliveira [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T16:52:39Z
dc.date.available2019-10-06T16:52:39Z
dc.date.issued2018-12-01
dc.description.abstractBackground: Photosensitizers in nanocarriers have been investigated for antimicrobial Photodynamic Therapy (aPDT). However, most studies are focused against microorganisms in planktonic or monospecies biofilm. Thus, this in vitro study evaluated the effect of aPDT using Chloroaluminium phthalocyanine (ClAlPc) in cationic nanoemulsion (NE) against Candida albicans, Candida glabrata and Streptococcus mutans grown as multispecies biofilm. Methods: Standard suspensions of each microorganism were added into wells of a microtiter plate for biofilm growth for 48 h in a candle jar. The biofilms were incubated with ClAlPc in cationic NE at 31.8 μM for 30 min and illuminated with red light fluence of 39.3 J/cm2 (P+L+ group). Additional samples were treated only with photosensitizer (P+L-) or red light (P-L+) or neither (P-L-, control group). aPDT efficacy was assessed by colony quantification, biofilm's metabolic activity, total biomass, and confocal microscopy. Data were analyzed by ANOVA/Welch and post-hoc Tukey/Games-Howell tests (α = 0.05). Results: aPDT (P+L+) reduced the colony count in 1.30 to 2.24 lg10 and the metabolic activity in 53.7% compared with the control group (P-L-). The total biomass showed no statistical difference among the groups. The confocal microscopy analyzes showed uptake of the PS in the biofilm, and dead cells were observed in the biofilm treated with aPDT. Conclusion: aPDT mediated by ClAlPc in cationic NE promoted photoinactivation of the multispecies biofilm, which was confirmed by colony quantification, metabolic activity, and confocal microscopy. However, the total biomass of the biofilm was not affected by the treatment.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationCenter of Nanotechnology and Tissue Engineers Photobiology and Photomedicine Research Group FFCLRP—São Paulo University
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/07276-1
dc.format.extent212-219
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2018.10.005
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 24, p. 212-219.
dc.identifier.doi10.1016/j.pdpdt.2018.10.005
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.lattes6259985899069498
dc.identifier.orcid0000-0002-9575-7625
dc.identifier.scopus2-s2.0-85054848814
dc.identifier.urihttp://hdl.handle.net/11449/189802
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntimicrobial photodynamic therapy
dc.subjectBacteria
dc.subjectBiofilm
dc.subjectFungi
dc.subjectNanocarriers
dc.subjectPhotochemotherapy
dc.titleEffect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilmen
dc.typeArtigo
unesp.author.lattes8867670539105403[4]
unesp.author.lattes6259985899069498(5)
unesp.author.orcid0000-0002-9231-1994[4]
unesp.author.orcid0000-0002-9575-7625(5)

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