Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells

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dc.contributor.authorMenoli, RCRN
dc.contributor.authorMantovani, M. S.
dc.contributor.authorRibeiro, Lúcia Regina [UNESP]
dc.contributor.authorSpeit, G.
dc.contributor.authorJordao, B. Q.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Ulm
dc.date.accessioned2014-05-20T13:37:05Z
dc.date.available2014-05-20T13:37:05Z
dc.date.issued2001-09-20
dc.description.abstractAgaricus blazei Murrill, a native mushroom in Brazil, has been widely consumed in different parts of the world due to its medicinal power. Its anticarcinogenic activity has been shown in experimental animals, and antimutagenic activity has been demonstrated only in Salmonella. In this work, the multagenic and antimutagenic activities of mushroom teas of strains AB96/07, AB96/09 and AB97/11 were evaluated in Chinese hamster V79 cells, using the comet assay and the micronucleus test. The cells were treated with three different concentrations (0.05, 0.1 and 0.15) of teas prepared from a 2.5% aqueous solution, under three different temperatures: (1) room (20-25 degreesC); (2) ice-cold (2-8 degreesC); and (3) warm (60 degreesC). The teas were applied in co-, pre- and post-treatments in combination with the mutagen methyl methanesulfonate (MMS; 1.6 x 10(-4) and 4 x 10(-4) M). The duration of the treatment was 1 h in the comet assay and 2 h in the micronucleus test. The results showed that the mushroom was not mutagenic itself. Nevertheless, the mushroom is an efficient antimutagen against the induction of micronuclei by MMS in all concentrations and preparations tested. The observed reductions in the frequencies of micronuclei ranged from 61.5 (room temperature 0.1% tea in post-treatment) to 110.3% (co-treatment with warm and ice-cold 0.15% tea). In the comet assay, the antimutagenic activity was detected only when the cells were pre-treated with the following teas: warm 0.1 and 0.15%, room temperature 0.05% and ice-cold 0.1%. The results indicate that the mushroom A. blazei extracts are antimutagenic when tested in V79 cells. (C) 2001 Elsevier B.V. B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Londrina, Dept Biol Geral, BR-86051990 Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Patol, Botucatu, SP, Brazil
dc.description.affiliationUniv Ulm, Abt Med Genet, Ulm, Germany
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Patol, Botucatu, SP, Brazil
dc.format.extent5-13
dc.identifierhttp://dx.doi.org/10.1016/S1383-5718(01)00227-3
dc.identifier.citationMutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 496, n. 1-2, p. 5-13, 2001.
dc.identifier.doi10.1016/S1383-5718(01)00227-3
dc.identifier.issn1383-5718
dc.identifier.urihttp://hdl.handle.net/11449/12802
dc.identifier.wosWOS:000170481900002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMutation Research: Genetic Toxicology and Environmental Mutagenesis
dc.relation.ispartofjcr1.996
dc.relation.ispartofsjr0,747
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAgaricus blazei Murrillpt
dc.subjectantimutagenesispt
dc.subjectmicronucleus testpt
dc.subjectcomet assaypt
dc.subjectV79 cellspt
dc.subjectmethyl methanesulfonatept
dc.titleAntimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0001-5268-6508[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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