Interaction between estradiol and 5-HT1A receptors in the median raphe nucleus on acquisition of aversive information and association to the context in ovariectomized rats

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dc.contributor.authorAndrade, Telma Gonçalves Carneiro Spera de [UNESP]
dc.contributor.authorSilva, João Victor dos Santos [UNESP]
dc.contributor.authorBatistela, Matheus Fitipaldi [UNESP]
dc.contributor.authorFrei, Fernando [UNESP]
dc.contributor.authorSant'Ana, Ana Beatriz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:32:45Z
dc.date.available2018-12-11T17:32:45Z
dc.date.issued2017-12-01
dc.description.abstractThe median raphe nucleus (MRN) is related to stress resistance and defensive responses, a crucial source of serotonergic neurons that project to prosencephalic structures related to stress and anxiety. Estrogen receptors were identified in this mesencephalic structure. It is possible that the estrogen action is related to serotonin effect on somatodendritic 5-HT1A receptors, inhibiting the function of serotonergic neurons and thus preventing of the stress effect and inducing anxiolysis. So, in order to evaluate these aspects, female Wistar rats were ovariectomized and 21 days later were given a direct microinjection of estradiol benzoate (EB) (1200 ng) into the MRN, preceded by microinjections of saline or WAY100.635 (100 ng), a 5-HT1A receptor antagonist. Immediately after the two microinjections, the ovariectomized rats were conditioned with an aversive event (foot shock) session in a Skinner box. Twenty-four hours later, they were exposed to the same context in a test session for 5 min for behavioral assessment: freezing, rearing, locomotion, grooming, and autonomic responses (fecal boluses and micturition). EB microinjection in the MRN prior to the exposure of animals to the foot shocks in the conditioning session did not alter their behavior in this session, but neutralized the association of the aversive experience to the context: there was a decrease in the expression of freezing and an increased rearing activity in the test session. This effect was reversed by prior microinjection of WAY100.635. In conclusion, EB acted on serotonergic neurons in the MRN of the ovariectomized rats, impairing the association of the aversive experience to the context, by co-modulating the functionality of somatodendritic 5-HT1A.en
dc.description.affiliationUNESP – Univ Estadual Paulista FCL Department of Biological Science, Avenida Dom Antonio, 2100
dc.description.affiliationLaboratory of Physiology UNESP
dc.description.affiliationUnespUNESP – Univ Estadual Paulista FCL Department of Biological Science, Avenida Dom Antonio, 2100
dc.description.affiliationUnespLaboratory of Physiology UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/06414-3
dc.format.extent80-88
dc.identifierhttp://dx.doi.org/10.1016/j.ynstr.2017.05.003
dc.identifier.citationNeurobiology of Stress, v. 7, p. 80-88.
dc.identifier.doi10.1016/j.ynstr.2017.05.003
dc.identifier.file2-s2.0-85020247624.pdf
dc.identifier.issn2352-2895
dc.identifier.scopus2-s2.0-85020247624
dc.identifier.urihttp://hdl.handle.net/11449/178929
dc.language.isoeng
dc.relation.ispartofNeurobiology of Stress
dc.relation.ispartofsjr2,404
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject5-HT1A receptors
dc.subjectAnxiety
dc.subjectContextual conditioning
dc.subjectEstradiol benzoate
dc.subjectMedian raphe nucleus
dc.subjectOvariectomized rats
dc.subjectWAY100.635
dc.titleInteraction between estradiol and 5-HT1A receptors in the median raphe nucleus on acquisition of aversive information and association to the context in ovariectomized ratsen
dc.typeArtigo

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