Aerobiology of the Wheat Blast Pathogen: Inoculum Monitoring and Detection of Fungicide Resistance Alleles

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dc.contributor.authorVicentini, Samara Nunes Campos [UNESP]
dc.contributor.authorHawkins, Nichola J.
dc.contributor.authorKing, Kevin M.
dc.contributor.authorMoreira, Silvino Intra [UNESP]
dc.contributor.authorde Paiva Custódio, Adriano Augusto
dc.contributor.authorLeite Júnior, Rui Pereira
dc.contributor.authorPortalanza, Diego
dc.contributor.authorGarcés-Fiallos, Felipe Rafael
dc.contributor.authorKrug, Loane Dantas [UNESP]
dc.contributor.authorWest, Jonathan S.
dc.contributor.authorFraaije, Bart A.
dc.contributor.authorDe Jesus Júnior, Waldir Cintra
dc.contributor.authorCeresini, Paulo Cezar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionNIAB
dc.contributor.institutionRothamsted Research
dc.contributor.institutionIDR–Paraná/IAPAR
dc.contributor.institutionUniversidad Agraria del Ecuador (UAE)
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.contributor.institutionTechnical University of Manabí
dc.contributor.institutionWageningen University & Research
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2023-07-29T16:15:04Z
dc.date.available2023-07-29T16:15:04Z
dc.date.issued2023-05-01
dc.description.abstractWheat blast, caused by the ascomycetous fungus Pyricularia oryzae Triticum lineage (PoTl), is mainly controlled by fungicide use, but resistance to the main fungicide groups—sterol demethylase (DMI), quinone outside (QoI), and succinate dehydrogenase inhibitors (SDHI)—has been reported in Brazil. In order to rationalize fungicide inputs (e.g., choice, timing, dose-rate, spray number, and mixing/alternation) for managing wheat blast, we describe a new monitoring tool, enabling the quantitative measurement of pathogen’s inoculum levels and detection of fungicide resistance alleles. Wheat blast airborne spores (aerosol populations) were monitored at Londrina in Paraná State, a major wheat cropping region in Brazil, using an automated high-volume cyclone coupled with a lab-based quantitative real-time PCR (qPCR) assay. The objectives of our study were as follows: (1) to monitor the amount of PoTl airborne conidia during 2019–2021 based on DNA detection, (2) to reveal the prevalence of QoI resistant (QoI-R) cytochrome b alleles in aerosol populations of wheat blast, and (3) to determine the impact of weather on the dynamics of wheat blast aerosol populations and spread of QoI resistant alleles. PoTl inoculum was consistently detected in aerosols during the wheat cropping seasons from 2019 to 2021, but amounts varied significantly between seasons, with highest amounts detected in 2019. High peaks of PoTl DNA were also continuously detected during the off-season in 2020 and 2021. The prevalence of QoI resistant (QoI-R) cytochrome b G143A alleles in aerosol populations was also determined for a subset of 10 PoTl positive DNA samples with frequencies varying between 10 and 91% using a combination of PCR-amplification and SNP detection pyrosequencing. Statistically significant but low correlations were found between the levels of pathogen and the weather variables. In conclusion, for wheat blast, this system provided prior detection of airborne spore levels of the pathogen and of the prevalence of fungicide resistance alleles.en
dc.description.affiliationDepartment of Crop Protection Agricultural Engineering and Soil Sao Paulo State University UNESP, SP
dc.description.affiliationNational Institute of Agricultural Botany NIAB
dc.description.affiliationProtecting Crops and Environment Rothamsted Research
dc.description.affiliationParaná Agricultural Development Institute IDR–Paraná/IAPAR, PR
dc.description.affiliationCarrera de Ingeniería Ambiental Facultad de Ciencias Agrarias Instituto de Investigación “Ing. Jacobo Bucaram Ortiz Ph.D” Universidad Agraria del Ecuador (UAE), Avenida 25 de Julio, Guayas
dc.description.affiliationCenter of Natural and Exact Sciences Department of Physics Federal University of Santa Maria (UFSM), Avenida Roraima 1000, RS
dc.description.affiliationLaboratory of Phytopathology Faculty of Agronomic Engineering Technical University of Manabí, Experimental Campus La Teodomira
dc.description.affiliationBU Biointeractions & Plant Health Wageningen Plant Research Wageningen University & Research
dc.description.affiliationCenter of Natural Science Federal University of São Carlos UFScar, Lagoa do Sino Campus, SP
dc.description.affiliationUnespDepartment of Crop Protection Agricultural Engineering and Soil Sao Paulo State University UNESP, SP
dc.description.sponsorshipRothamsted Research
dc.identifierhttp://dx.doi.org/10.3390/agronomy13051238
dc.identifier.citationAgronomy, v. 13, n. 5, 2023.
dc.identifier.doi10.3390/agronomy13051238
dc.identifier.issn2073-4395
dc.identifier.scopus2-s2.0-85160433851
dc.identifier.urihttp://hdl.handle.net/11449/249999
dc.language.isoeng
dc.relation.ispartofAgronomy
dc.sourceScopus
dc.subjectairborne spores
dc.subjectepidemic predictors
dc.subjectintegrated disease management
dc.subjectPyricularia oryzae Triticum lineage
dc.titleAerobiology of the Wheat Blast Pathogen: Inoculum Monitoring and Detection of Fungicide Resistance Allelesen
dc.typeArtigo
unesp.author.orcid0000-0003-4693-8806[4]
unesp.author.orcid0000-0002-1932-8060[5]
unesp.author.orcid0000-0001-5275-0741[7]
unesp.author.orcid0000-0002-1795-4439[8]
unesp.author.orcid0000-0002-5211-2405[10]
unesp.author.orcid0000-0001-8176-2258[11]
unesp.author.orcid0000-0003-2381-2792[13]
unesp.departmentFitossanidade, Engenharia Rural e Solos - FEISpt

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Ilha Solteira - FEIS - Faculdade de Engenharia