Neonatal BCG Immunization Followed by DNAhsp65 Boosters: Highly Immunogenic but not Protective Against Tuberculosis - a Paradoxical Effect of the Vector?

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dc.contributor.authorPelizon, A. C. [UNESP]
dc.contributor.authorMartins, D. R. [UNESP]
dc.contributor.authorZorzella-Pezavento, S. F. G. [UNESP]
dc.contributor.authorSeger, J. [UNESP]
dc.contributor.authorJustulin, L. A. [UNESP]
dc.contributor.authorda Fonseca, D. M.
dc.contributor.authorSantos, R. R.
dc.contributor.authorMasson, A. P.
dc.contributor.authorSilva, C. L.
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:51:14Z
dc.date.available2014-05-20T13:51:14Z
dc.date.issued2010-02-01
dc.description.abstractA new tuberculosis vaccine is urgently needed. Prime-boost strategies are considered very promising and the inclusion of BCG is highly desirable. In this investigation, we tested the protective efficacy of BCG delivered in the neonatal period followed by boosters in the adult phase with a DNA vaccine containing the hsp65 gene from Mycobacterium leprae (pVAXhsp65). Immune responses were characterized by serum anti-hsp65 antibody levels and IFN-gamma and IL-5 production by the spleen. Amounts of these cytokines were also determined in lung homogenates. Protective efficacy was established by the number of colony-forming units (CFU) and histopathological analysis of the lungs after challenge with Mycobacterium tuberculosis. Immunization with BCG alone triggered a significant reduction of CFU in the lungs and also clearly preserved the pulmonary parenchyma. BCG priming also increased the immunogenicity of pVAXhsp65. However, boosters with pVAXhsp65 or the empty vector abolished the protective efficacy of BCG. Also, higher IL-5 levels were produced by spleen and lungs after DNA boosters. These results demonstrated that neonatal BCG immunization followed by DNAhsp65 boosters is highly immunogenic but is not protective against tuberculosis.en
dc.description.affiliationSão Paulo State Univ UNESP, Dept Microbiol Immunol, Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dept Morphol, Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Biochem & Immunol, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Microbiol Immunol, Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Morphol, Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 03/06348-7
dc.format.extent63-69
dc.identifierhttp://dx.doi.org/10.1111/j.1365-3083.2009.02352.x
dc.identifier.citationScandinavian Journal of Immunology. Malden: Wiley-blackwell Publishing, Inc, v. 71, n. 2, p. 63-69, 2010.
dc.identifier.doi10.1111/j.1365-3083.2009.02352.x
dc.identifier.fileWOS000273688300001.pdf
dc.identifier.issn0300-9475
dc.identifier.lattes4977572416129527
dc.identifier.urihttp://hdl.handle.net/11449/18290
dc.identifier.wosWOS:000273688300001
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofScandinavian Journal of Immunology
dc.relation.ispartofjcr2.314
dc.relation.ispartofsjr0,891
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleNeonatal BCG Immunization Followed by DNAhsp65 Boosters: Highly Immunogenic but not Protective Against Tuberculosis - a Paradoxical Effect of the Vector?en
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
unesp.author.lattes4977572416129527[10]
unesp.author.orcid0000-0003-4557-3331[10]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB