The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers

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dc.contributor.authorVieira, José Cavalcante Souza [UNESP]
dc.contributor.authorBraga, Camila Pereira
dc.contributor.authorQueiroz, João Vitor de [UNESP]
dc.contributor.authorCavecci-Mendonça, Bruna [UNESP]
dc.contributor.authorOliveira, Grasieli de [UNESP]
dc.contributor.authorFreitas, Natália Galvão de [UNESP]
dc.contributor.authorFernandes, Ana Angélica Henrique [UNESP]
dc.contributor.authorFernandes, Mileni da Silva
dc.contributor.authorBuzalaf, Marília Afonso Rabelo
dc.contributor.authorAdamec, Jiri
dc.contributor.authorZara, Luiz Fabrício
dc.contributor.authorPadilha, Pedro de Magalhães [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Nebraska (UNL)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionCollege of Planaltina
dc.date.accessioned2023-07-29T16:02:50Z
dc.date.available2023-07-29T16:02:50Z
dc.date.issued2023-03-01
dc.description.abstractExposure to mercury can interfere with the expression of proteins and enzymes, compromise important pathways, such as apoptosis and glucose metabolism, and even induce the expression of metallothioneins. In this study, analytical techniques were used to determine the concentration of total mercury (THg) in muscle and liver tissue, protein pellets, and spots [using graphite furnace atomic absorption spectrometry (GFAAS)], and molecular techniques were used to identify metalloproteins present in mercury-associated protein spots. Thirty individuals from three different fish species, Cichla sp. (n = 10), Brachyplatystoma filamentosum (n = 10), and Semaprochilodus sp. (n = 10) from the Brazilian Amazon were used. Oxidative stress indicators [such as glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), a marker of lipid peroxidation (LPO)] and the possible expression of metallothioneins in muscle and liver tissues were investigated. The two piscivorous species, Cichla sp. and B. filamentosum, presented the highest concentrations of mercury in their hepatic tissue, 1219 ± 15.00 and 1044 ± 13.6 μg kg−1, respectively, and in their muscle tissue, 101 ± 1.30 μg kg−1 and 87.4 ± 0.900 μg kg−1, respectively. The non-carnivorous species Semaprochilodus sp. had comparatively low concentrations of mercury in both its hepatic (852 ± 11.1 μg kg−1) and muscle (71.4 ± 0.930 μg kg−1) tissues. The presence of mercury was identified in 24 protein spots using GFAAS; concentrations ranged from 11.5 to 787 μg kg−1, and mass spectrometry identified 21 metal-binding proteins. The activities of GSH-Px, CAT, and SOD, related to oxidative stress, decreased proportionally as tissue Hg concentrations increased, while the levels of LPO markers increased, indicating the presence of stress. Our study results demonstrate possible mercury interference in oxidative stress markers (GSH-Px, CAT, SOD, and LPO), in addition to the identification of 21 metal-binding proteins as possible biomarkers of mercury exposure in fish.en
dc.description.affiliationSão Paulo State University (UNESP) Institute of Biosciences, São Paulo
dc.description.affiliationUniversity of Nebraska (UNL)
dc.description.affiliationSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, SP
dc.description.affiliationUniversity of São Paulo (USP)
dc.description.affiliationUniversity of Brasília (UNB) College of Planaltina
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Biosciences, São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, SP
dc.identifierhttp://dx.doi.org/10.1016/j.chemosphere.2023.137779
dc.identifier.citationChemosphere, v. 316.
dc.identifier.doi10.1016/j.chemosphere.2023.137779
dc.identifier.issn1879-1298
dc.identifier.issn0045-6535
dc.identifier.scopus2-s2.0-85146161032
dc.identifier.urihttp://hdl.handle.net/11449/249549
dc.language.isoeng
dc.relation.ispartofChemosphere
dc.sourceScopus
dc.subjectMercury biomarkers
dc.subjectMetal-binding protein
dc.subjectMetalloproteomics
dc.subjectMetallothionein
dc.titleThe effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkersen
dc.typeArtigo
unesp.author.orcid0000-0001-5447-8913[4]
unesp.author.orcid0000-0002-5985-3951[9]
unesp.author.orcid0000-0003-4899-4406[10]
unesp.author.orcid0000-0001-6110-2527[11]

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Artigos - Química e Bioquímica - IBB