Ferric iron uptake genes are differentially expressed in the presence of copper sulfides in Acidithiobacillus ferrooxidans strain LR

Página do item simplificado
dc.contributor.authorFerraz, Lucio F. C.
dc.contributor.authorVerde, Leandro C. L.
dc.contributor.authorVicentini, Renato
dc.contributor.authorFelicio, Ana P. [UNESP]
dc.contributor.authorRibeiro, Marcelo L.
dc.contributor.authorAlexandrino, Fabiana
dc.contributor.authorNovo, Maria T. M.
dc.contributor.authorGarcia, Oswaldo [UNESP]
dc.contributor.authorRigden, Daniel J.
dc.contributor.authorOttoboni, Laura M. M.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniv Liverpool
dc.contributor.institutionUniv Sao Francisco
dc.date.accessioned2014-05-20T14:17:40Z
dc.date.available2014-05-20T14:17:40Z
dc.date.issued2011-03-01
dc.description.abstractAcidithiobacillus ferrooxidans is one of the most widely used microorganisms in bioleaching operations to recover copper from low-grade copper sulfide ores. This work aimed to investigate the relative expression of genes related to the iron uptake system when A. ferrooxidans LR was maintained in contact with chalcopyrite or bornite as the sole energy source. Real-time quantitative PCR analysis revealed that the presence of bornite had no effect on the expression of seven genes related to the siderophore-mediated Fe(III) uptake system, while in the presence of chalcopyrite the expression of the genes was up-regulated. Bioinformatic analysis of the genomic region where these genes were found revealed the existence of three new putative DNA-binding sequences for the ferric iron uptake transcriptional regulator (Fur). Electrophoretic mobility shift assays demonstrated that a purified A. ferrooxidans His-tagged Fur protein was able to bind in vitro to each of these putative Fur boxes, suggesting that Fur regulated the expression of these genes. The expression of fur and two known Fur-regulated genes, mntH and dsrK, was also investigated in the presence of chalcopyrite. While the expression of fur and mntH was up-regulated, the expression of dsrK was down-regulated. The low amount of ferrous iron in the medium was probably responsible for the up-regulation of fur and the genes related to the siderophore-mediated Fe(III) uptake system when A. ferrooxidans LR was kept in the presence of chalcopyrite. A homology model of the A. ferrooxidans Fur was constructed and revealed that the putative DNA-binding surface presents conserved positively charged residues, supporting a previously suggested mode of interaction with DNA. The up-regulation of fur and the siderophore-mediated Fe(III) uptake genes, and the down-regulation of dsrK suggest that in the presence of chalcopyrite Fur acts as a transcription inducer and repressor.en
dc.description.affiliationUniv Estadual Campinas UNICAMP, Ctr Biol Mol & Engn Genet CBMEG, BR-13083875 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniversidade Federal de São Carlos (UFSCar), Ctr Ciencias Biol & da Saude, Dept Genet & Evolu, BR-13565905 São Carlos, SP, Brazil
dc.description.affiliationUniv Liverpool, Inst Integrat Biol, Liverpool L69 7ZB, Merseyside, England
dc.description.affiliationUniv Sao Francisco, Unidade Integrada Farmacol & Gastroenterol, BR-12916900 Braganca Paulista, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil
dc.description.sponsorshipCompany Vale
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Apoio à Ciência Tecnologia e Educação (FACTE)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent609-617
dc.identifierhttp://dx.doi.org/10.1007/s10482-010-9533-2
dc.identifier.citationAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. Dordrecht: Springer, v. 99, n. 3, p. 609-617, 2011.
dc.identifier.doi10.1007/s10482-010-9533-2
dc.identifier.issn0003-6072
dc.identifier.urihttp://hdl.handle.net/11449/25298
dc.identifier.wosWOS:000287450200018
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
dc.relation.ispartofjcr1.588
dc.relation.ispartofsjr0,834
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAcidithiobacillus ferrooxidansen
dc.subjectCopper sulfidesen
dc.subjectSiderophore-mediated Fe(III) uptake genesen
dc.titleFerric iron uptake genes are differentially expressed in the presence of copper sulfides in Acidithiobacillus ferrooxidans strain LRen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Bioquímica e Tecnologia - IQ