Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules
dc.contributor.author | Ben Aissa, A. | |
dc.contributor.author | Herrera-Chacon, A. | |
dc.contributor.author | Pupin, R. R. [UNESP] | |
dc.contributor.author | Sotomayor, M. D.P.T. [UNESP] | |
dc.contributor.author | Pividori, M. I. | |
dc.contributor.institution | Universitat Autònoma de Barcelona | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2018-12-11T17:07:34Z | |
dc.date.available | 2018-12-11T17:07:34Z | |
dc.date.issued | 2017-02-15 | |
dc.description.abstract | Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets. | en |
dc.description.affiliation | Grup de Sensors i Biosensors Departament de Química Universitat Autònoma de Barcelona | |
dc.description.affiliation | UNESP – Univ Estadual Paulista Institute of Chemistry Department of Analytical Chemistry | |
dc.description.affiliationUnesp | UNESP – Univ Estadual Paulista Institute of Chemistry Department of Analytical Chemistry | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Ministerio de Economía y Competitividad | |
dc.description.sponsorshipId | CNPq: 151525/2013-7 | |
dc.description.sponsorshipId | CNPq: 303979/2012-7 | |
dc.description.sponsorshipId | CNPq: 4004759/2012-4 | |
dc.description.sponsorshipId | Ministerio de Economía y Competitividad: BIO2013-41242-R | |
dc.format.extent | 101-108 | |
dc.identifier | http://dx.doi.org/10.1016/j.bios.2016.07.096 | |
dc.identifier.citation | Biosensors and Bioelectronics, v. 88, p. 101-108. | |
dc.identifier.doi | 10.1016/j.bios.2016.07.096 | |
dc.identifier.file | 2-s2.0-84994583502.pdf | |
dc.identifier.issn | 1873-4235 | |
dc.identifier.issn | 0956-5663 | |
dc.identifier.scopus | 2-s2.0-84994583502 | |
dc.identifier.uri | http://hdl.handle.net/11449/173750 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biosensors and Bioelectronics | |
dc.relation.ispartofsjr | 2,373 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | biotinylated biomolecules | |
dc.subject | immunomagnetic separation | |
dc.subject | magnetic molecularly-imprinted polymer | |
dc.subject | magnetic particles | |
dc.subject | magneto-actuated immunoassay | |
dc.title | Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules | en |
dc.type | Artigo |
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