Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules

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dc.contributor.authorBen Aissa, A.
dc.contributor.authorHerrera-Chacon, A.
dc.contributor.authorPupin, R. R. [UNESP]
dc.contributor.authorSotomayor, M. D.P.T. [UNESP]
dc.contributor.authorPividori, M. I.
dc.contributor.institutionUniversitat Autònoma de Barcelona
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:07:34Z
dc.date.available2018-12-11T17:07:34Z
dc.date.issued2017-02-15
dc.description.abstractMagnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.en
dc.description.affiliationGrup de Sensors i Biosensors Departament de Química Universitat Autònoma de Barcelona
dc.description.affiliationUNESP – Univ Estadual Paulista Institute of Chemistry Department of Analytical Chemistry
dc.description.affiliationUnespUNESP – Univ Estadual Paulista Institute of Chemistry Department of Analytical Chemistry
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipMinisterio de Economía y Competitividad
dc.description.sponsorshipIdCNPq: 151525/2013-7
dc.description.sponsorshipIdCNPq: 303979/2012-7
dc.description.sponsorshipIdCNPq: 4004759/2012-4
dc.description.sponsorshipIdMinisterio de Economía y Competitividad: BIO2013-41242-R
dc.format.extent101-108
dc.identifierhttp://dx.doi.org/10.1016/j.bios.2016.07.096
dc.identifier.citationBiosensors and Bioelectronics, v. 88, p. 101-108.
dc.identifier.doi10.1016/j.bios.2016.07.096
dc.identifier.file2-s2.0-84994583502.pdf
dc.identifier.issn1873-4235
dc.identifier.issn0956-5663
dc.identifier.scopus2-s2.0-84994583502
dc.identifier.urihttp://hdl.handle.net/11449/173750
dc.language.isoeng
dc.relation.ispartofBiosensors and Bioelectronics
dc.relation.ispartofsjr2,373
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectbiotinylated biomolecules
dc.subjectimmunomagnetic separation
dc.subjectmagnetic molecularly-imprinted polymer
dc.subjectmagnetic particles
dc.subjectmagneto-actuated immunoassay
dc.titleMagnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomoleculesen
dc.typeArtigo

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