Changes in calsequestrin, TNF-alpha, TGF-beta and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles

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dc.contributor.authorMaranhao, Juliana Barros
dc.contributor.authorMoreira, Drielen de Oliveira
dc.contributor.authorMauricio, Adriana Fogagnolo
dc.contributor.authorCarvalho, Samara Camacari de
dc.contributor.authorFerretti, Renato [UNESP]
dc.contributor.authorPereira, Juliano Alves
dc.contributor.authorSanto Neto, Humberto
dc.contributor.authorMarques, Maria Julia
dc.contributor.institutionUniv Pernambuco UPE
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:19:06Z
dc.date.available2018-11-26T16:19:06Z
dc.date.issued2015-10-01
dc.description.abstractIn Duchenne muscular dystrophy (DMD), the search for new biomarkers to follow the evolution of the disease is of fundamental importance in the light of the evolving gene and pharmacological therapies. In addition to the lack of dystrophin, secondary events including changes in calcium levels, inflammation and fibrosis greatly contribute to DMD progression and the molecules involved in these events may represent potential biomarkers. In this study, we performed a comparative evaluation of the progression of dystrophy within muscles that are differently affected by dystrophy (diaphragm; DIA and quadriceps; QDR) or spared (intrinsic laryngeal muscles) using the mdx mice model of DMD. We assessed muscle levels of calsequestrin (calcium-related protein), tumour necrosis factor (TNF-alpha; pro-inflammatory cytokine), tumour growth factor (TGF-beta; pro-fibrotic factor) and MyoD (muscle proliferation) vs. histopathology at early (1 and 4 months of age) and late (9 months of age) stages of dystrophy. Fibrosis was the primary feature in the DIA of mdx mice (9 months: 32% fibrosis), which was greater than in the QDR (9 months: 0.6% fibrosis). Muscle regeneration was the primary feature in the QDR (9 months: 90% of centrally nucleated fibres areas vs. 33% in the DIA). The QDR expressed higher levels of calsequestrin than the DIA. Laryngeal muscles showed normal levels of TNF-alpha, TGF-beta and MyoD. A positive correlation between histopathology and cytokine levels was observed only in the diaphragm, suggesting that TNF-alpha and TGF-beta serve as markers of dystrophy primarily for the diaphragm.en
dc.description.affiliationUniv Pernambuco UPE, Recife, PE, Brazil
dc.description.affiliationUniv Estadual Campinas UNICAMP, Inst Biol, Dept Biol Estrut & Func, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 04/15526-9
dc.description.sponsorshipIdFAPESP: 08/58491-1
dc.description.sponsorshipIdFAPESP: 11/51697-6
dc.description.sponsorshipIdFAPESP: 14/04782-6
dc.description.sponsorshipIdCNPq: 303320/2013-3
dc.description.sponsorshipIdCNPq: 302831/2013-4
dc.description.sponsorshipIdCNPq: 142282/2012-0
dc.description.sponsorshipIdFAPESP: 2012/03498-7
dc.description.sponsorshipIdFAPESP: 2012/13577-1
dc.description.sponsorshipIdFAPESP: 2012/15492-3
dc.format.extent285-293
dc.identifierhttp://dx.doi.org/10.1111/iep.12142
dc.identifier.citationInternational Journal Of Experimental Pathology. Hoboken: Wiley-blackwell, v. 96, n. 5, p. 285-293, 2015.
dc.identifier.doi10.1111/iep.12142
dc.identifier.issn0959-9673
dc.identifier.lattes3681613160086175
dc.identifier.orcid0000-0003-3944-1906
dc.identifier.urihttp://hdl.handle.net/11449/161091
dc.identifier.wosWOS:000367324300002
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInternational Journal Of Experimental Pathology
dc.relation.ispartofsjr0,712
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcalsequestrin
dc.subjectdystrophy
dc.subjectmdx
dc.subjecttumour growth factor-beta
dc.subjecttumour necrosis factor-alpha
dc.titleChanges in calsequestrin, TNF-alpha, TGF-beta and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal musclesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes3681613160086175[5]
unesp.author.orcid0000-0003-3944-1906[5]

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