Effects of mineral trioxide aggregate, Biodentine (TM) and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth

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dc.contributor.authorAraujo, Leandro Borges
dc.contributor.authorCosme-Silva, Leopoldo
dc.contributor.authorFernandes, Ana Paula
dc.contributor.authorOliveira, Thais Marchini de
dc.contributor.authorCavalcanti, Bruno das Neves
dc.contributor.authorGomes Filho, Joao Eduardo [UNESP]
dc.contributor.authorSakai, Vivien Thiemy
dc.contributor.institutionUniv Fed Alfenas
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Iowa
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-11T06:51:30Z
dc.date.available2020-12-11T06:51:30Z
dc.date.issued2018-01-01
dc.description.abstractObjective: The aim of the study was to evaluate the effects of the capping materials mineral trioxide aggregate (MTA), calcium hydroxide (CH) and Biodentine (TM) (BD) on stem cells from human exfoliated deciduous teeth (SHED) in vitro. Material and Methods: SHED were cultured for 1 - 7 days in medium conditioned by incubation with MTA, BD or CH (1 mg/mL), and tested for viability (MTT assay) and proliferation (SRB assay). Also, the migration of serum-starved SHED towards conditioned media was assayed in companion plates, with 8 mm-pore-sized membranes, for 24 h. Gene expression of dentin matrix protein-1 (DMP-1) was evaluated by reverse-transcription polymerase chain reaction. Regular culture medium with 10% FBS (without conditioning) and culture medium supplemented with 20% FBS were used as controls. Results: MTA, CH and BD conditioned media maintained cell viability and allowed continuous SHED proliferation, with CH conditioned medium causing the highest positive effect on proliferation at the end of the treatment period (compared with BD and MTA) (p<0.05). In contrast, we observed increased SHED migration towards BD and MTA conditioned media (compared with CH) (p<0.05). A greater amount of DMP-1 gene was expressed in MTA group compared with the other groups from day 7 up to day 21. Conclusion: Our results show that the three capping materials are biocompatible, maintain viability and stimulate proliferation, migration and differentiation in a key dental stem cell population.en
dc.description.affiliationUniv Fed Alfenas, Fac Odontol, Dept Clin & Cirurgia Alfenas, Alfenas, MG, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Odontol Bauru, Dept Odontopediatria Ortodontia & Saude Coletiva, Sao Paulo, Brazil
dc.description.affiliationUniv Iowa, Coll Dent, Iowa City, IA USA
dc.description.affiliationUniv Estadual Paulista, Dept Odontol Restauradora, Fac Odontol, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Odontol Restauradora, Fac Odontol, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPEMIG: APQ-00215-11
dc.description.sponsorshipIdCNPq: 471568/2011-2
dc.description.sponsorshipIdCAPES: 8881.068437/2014-1
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1590/1678-7757-2016-0629
dc.identifier.citationJournal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 26, 8 p., 2018.
dc.identifier.doi10.1590/1678-7757-2016-0629
dc.identifier.fileS1678-77572018000100417.pdf
dc.identifier.issn1678-7757
dc.identifier.scieloS1678-77572018000100417
dc.identifier.urihttp://hdl.handle.net/11449/197648
dc.identifier.wosWOS:000507906000001
dc.language.isoeng
dc.publisherUniv Sao Paulo Fac Odontologia Bauru
dc.relation.ispartofJournal Of Applied Oral Science
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBiomaterials
dc.subjectCell differentiations
dc.subjectDental pulp capping
dc.subjectStem cells
dc.subjectVital pulp therapy
dc.titleEffects of mineral trioxide aggregate, Biodentine (TM) and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teethen
dc.typeArtigo
dcterms.rightsHolderUniv Sao Paulo Fac Odontologia Bauru

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