Development and Validation of a Green Analytical Method of RP-HPLC for Quantification of Cefepime Hydrochloride in Pharmaceutical Dosage Form: Simple, Sensitive and Economic

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dc.contributor.authorRodrigues, Danilo Fernando [UNESP]
dc.contributor.authorNunes Salgado, Herida Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T17:06:27Z
dc.date.available2018-11-26T17:06:27Z
dc.date.issued2016-01-01
dc.description.abstractIntroduction: A green analytical method simple, rapid and economic was validated for the determination of cefepime hydrochloride and has been developed using reversed phase high performance liquid chromatography (RP-HPLC) in pharmaceutical preparations. Methods: The liquid chromatography method was carried out using as mobile phase a mixture of ethanol and water (55: 45, v/v), on column Luna C18 (250 x 4.6 mm; 5 mu m) at a flow rate of 0.5 mL min-1 with UV detection at 258 nm. Results: The method was validated according to ICH guidelines, presenting optimum linearity in the range 10-70 mu g mL(-1) (r = 1.0000) and retention time (tR) equal to 4.99 minutes. Statistical analyses were performed for the validated method, proving the safety and reliability of the results. Other important factors is that the method uses less amounts of organic solvent, produces low levels of waste and does not use buffer solution, minimizing effluent treatment, which contributes to the environment and implements methods aimed green chemistry, making economic for the industry. Conclusion: Thus, the method can be employed as an alternative in quality control routine analysis of pharmaceutical industry for quantification of cefepime hydrochloride lyophilized powder for solution for injection.en
dc.description.affiliationUniv Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPADC - UNESP (Sao Paulo, Brazil)
dc.format.extent306-314
dc.identifierhttp://dx.doi.org/10.2174/1573412912666151221210921
dc.identifier.citationCurrent Pharmaceutical Analysis. Sharjah: Bentham Science Publ Ltd, v. 12, n. 4, p. 306-314, 2016.
dc.identifier.doi10.2174/1573412912666151221210921
dc.identifier.issn1573-4129
dc.identifier.urihttp://hdl.handle.net/11449/161995
dc.identifier.wosWOS:000384722700004
dc.language.isoeng
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofCurrent Pharmaceutical Analysis
dc.relation.ispartofsjr0,224
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectGreen analytical method
dc.subjectcefepime hydrochloride
dc.subjectethanol
dc.subjectvalidation
dc.subjectquality control
dc.subjectRP-HPLC
dc.titleDevelopment and Validation of a Green Analytical Method of RP-HPLC for Quantification of Cefepime Hydrochloride in Pharmaceutical Dosage Form: Simple, Sensitive and Economicen
dc.typeArtigo
dcterms.rightsHolderBentham Science Publ Ltd
unesp.author.orcid0000-0003-0762-6199[1]

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