Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala (Fabricius, 1794)

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dc.contributor.authorFaraldo, Ana Carolina
dc.contributor.authorSá-Nunes, A.
dc.contributor.authorFaccioli, L. H.
dc.contributor.authorDel Bel, E. A.
dc.contributor.authorLello, E. [UNESP]
dc.contributor.institutionUnidade Acadêmica de Garanhuns
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUnidade Academica de Garanhuns
dc.date.accessioned2022-04-28T20:19:46Z
dc.date.available2022-04-28T20:19:46Z
dc.date.issued2007-09-25
dc.description.abstractAlthough insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected (SIL) larvae of the blowfly Chrysomya megacephala, using the NADPH diaphorase technique. Our findings revealed similar levels of NOS activity in muscle, fat body, Malpighian tubule, gut, and brain, suggesting that NO synthesis may not be involved in the immune response of these larval systems. These results were compared to many studies that recorded the involvement of NO in various physiological functions of insects.en
dc.description.affiliationUniversidade Federal Rural de Pernambuco Unidade Acadêmica de Garanhuns, Garanhuns, Pernambuco
dc.description.affiliationDepartamento de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto USP, Ribeirão Preto, SP
dc.description.affiliationDepartamento de Morfologia, Estomatologia e Fisiologia Faculdade de Odontologia de Ribeirão Preto USP, Ribeirão Preto, SP
dc.description.affiliationDepartamento de Morfologia Instituto de Biociências UNESP, Botucatu, São Paulo
dc.description.affiliationUniversidade Federal Rural de Pernambuco Unidade Academica de Garanhuns, Avenida Bom Pastor, s/n, 55.292-901, Garanhuns, PE
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biociências UNESP, Botucatu, São Paulo
dc.format.extent205-211
dc.identifier.citationBiocell, v. 31, n. 2, p. 205-211, 2007.
dc.identifier.issn0327-9545
dc.identifier.scopus2-s2.0-34548765457
dc.identifier.urihttp://hdl.handle.net/11449/224956
dc.language.isoeng
dc.relation.ispartofBiocell
dc.sourceScopus
dc.subjectBlowfly
dc.subjectChrysomya megacephala
dc.subjectNADPH-diaphorase
dc.subjectNitric oxide
dc.subjectNO synthase
dc.subjectTissues
dc.titleNitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala (Fabricius, 1794)en
dc.typeArtigo

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