Preliminary study on testicular germ cell isolation and transplantation in an endangered endemic species Brycon orbignyanus (Characiformes: Characidae)

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dc.contributor.authorde Siqueira-Silva, Diógenes Henrique [UNESP]
dc.contributor.authordos Santos Silva, Amanda Pereira [UNESP]
dc.contributor.authorda Silva Costa, Raphael [UNESP]
dc.contributor.authorSenhorini, José Augusto
dc.contributor.authorNinhaus-Silveira, Alexandre [UNESP]
dc.contributor.authorVeríssimo-Silveira, Rosicleire [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionChico Mendes Institute of Biodiversity Conservation
dc.date.accessioned2019-10-06T16:26:16Z
dc.date.available2019-10-06T16:26:16Z
dc.date.issued2019-01-01
dc.description.abstractWe aimed to develop a simplified protocol for transplantation of Brycon orbignyanus spermatogonial stem cells (SSCs) into Astyanax altiparanae testes. Brycon orbignyanus testes were enzymatically digested and SSC purified by a discontinuous density gradient. Endogenous spermatogenesis was suppressed in A. altiparanae using busulfan or by incubation at 35 °C water, and SSCs from B. orbignyanus labeled with PKH26 were injected into their testes via the urogenital papilla. Twenty-two hours post-transplantation, labeled spermatogonia were observed in A. altiparanae tubular lumen. After 7 days, spermatogonia proliferated in the epithelium, and 21 days post-transplantation, sperm was observed in the lumen. Of surviving host fish, nearly 67% of those treated with busulfan and 85% of those held in warm water showed labeled cells in host germinal epithelium. The present study standardized, by a simple and accessible method, germ cell transplantation between sexually mature Characiformes fish species. This is the first report of xenogenic SSC transplantation in this fish order.en
dc.description.affiliationCampus de Ilha Solteira Departament of Biology and Animal Science L.I.NEO – Laboratório de Ictiologia Neotropical UNESP – Univ. Estadual Paulista, Avenida Brasil Centro, 56
dc.description.affiliationCampus de São José do Rio Preto Post-Graduation Program in Animal Biology UNESP – Univ. Estadual Paulista, Rua Cristovão Colombo, Jardim Nazareth, 2265
dc.description.affiliationCampus de Ilha Solteira Departamento de Biologia e Zootecnia L.I.NEO - Laboratório de Ictiologia Neotropical UNESP –Univ. Estadual Paulista, Rua Monção, 226, Zona Norte
dc.description.affiliationCEPTA-ICMBIO - National Center for Research and Conservation of Continental Fish Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemesio Pereira de Godoy
dc.description.affiliationUnespCampus de Ilha Solteira Departament of Biology and Animal Science L.I.NEO – Laboratório de Ictiologia Neotropical UNESP – Univ. Estadual Paulista, Avenida Brasil Centro, 56
dc.description.affiliationUnespCampus de São José do Rio Preto Post-Graduation Program in Animal Biology UNESP – Univ. Estadual Paulista, Rua Cristovão Colombo, Jardim Nazareth, 2265
dc.description.affiliationUnespCampus de Ilha Solteira Departamento de Biologia e Zootecnia L.I.NEO - Laboratório de Ictiologia Neotropical UNESP –Univ. Estadual Paulista, Rua Monção, 226, Zona Norte
dc.identifierhttp://dx.doi.org/10.1007/s10695-019-00631-8
dc.identifier.citationFish Physiology and Biochemistry.
dc.identifier.doi10.1007/s10695-019-00631-8
dc.identifier.issn1573-5168
dc.identifier.issn0920-1742
dc.identifier.lattes2926053984914477
dc.identifier.orcid0000-0002-8298-5004
dc.identifier.scopus2-s2.0-85064455273
dc.identifier.urihttp://hdl.handle.net/11449/188991
dc.language.isoeng
dc.relation.ispartofFish Physiology and Biochemistry
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectFish chimera
dc.subjectGerm cell technology
dc.subjectSpermatogonial proliferation
dc.subjectSurrogate production
dc.titlePreliminary study on testicular germ cell isolation and transplantation in an endangered endemic species Brycon orbignyanus (Characiformes: Characidae)en
dc.typeArtigo
unesp.author.lattes9568814835325380[5]
unesp.author.lattes2926053984914477[6]
unesp.author.orcid0000-0003-0738-0862[5]
unesp.author.orcid0000-0002-8298-5004[6]

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Artigos - Biologia e Zootecnia - FEIS
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