Isolation and characterization of farm pig adipose tissue-derived mesenchymal stromal/stem cells

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dc.contributor.authorGarcia, G. A. [UNESP]
dc.contributor.authorOliveira, R. G. [UNESP]
dc.contributor.authorDariolli, R.
dc.contributor.authorRudge, M. V.C. [UNESP]
dc.contributor.authorBarbosa, A. M.P. [UNESP]
dc.contributor.authorFloriano, J. F. [UNESP]
dc.contributor.authorRibeiro-Paes, J. T. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto do Corac¸ão
dc.contributor.institutionIcahn School of Medicine at Mount Sinai
dc.date.accessioned2023-07-29T13:31:03Z
dc.date.available2023-07-29T13:31:03Z
dc.date.issued2022-01-01
dc.description.abstractAdipose tissue-derived mesenchymal stromal/stem cells (ASCs) are considered important tools in regenerative medicine and are being tested in several clinical studies. Porcine models are frequently used to obtain adipose tissue, due to the abundance of material and because they have immunological and physiological similarities with humans. However, it is essential to understand the effects and safe application of ASCs from pigs (pASCs) as an alternative therapy for diseases. Although minipigs are easy-to-handle animals that require less food and space, acquiring and maintaining them in a bioterium can be costly. Thus, we present a protocol for the isolation and proliferation of ASCs isolated from adipose tissue of farm pigs. Adipose tissue samples were extracted from the abdominal region of the animals. Because the pigs were not raised in a controlled environment, such as a bioterium, it was necessary to carry out rigorous procedures for disinfection. After this procedure, cells were isolated by mechanical dissociation and enzymatic digestion. A proliferation curve was performed and used to calculate the doubling time of the population. The characterization of pASCs was performed by immunophenotyping and cell differentiation in osteogenic and adipogenic lineages. The described method was efficient for the isolation and cultivation of pASCs, maintaining cellular attributes, such as surface antigens and multipotential differentiation during in vitro proliferation. This protocol presents the isolation and cultivation of ASCs from farm pig as an alternative for the isolation and cultivation of ASCs from minipigs, which require strictly controlled maintenance conditions and a more expensive process.en
dc.description.affiliationDepartamento de Biotecnologia Faculdade de Ciências e Letras Universidade Estadual Paulista, SP
dc.description.affiliationInstituto de Ciências Biomédicas Universidade de São Paulo, SP
dc.description.affiliationLaboratório de Genética e Cardiologia Molecular Instituto do Corac¸ão, SP
dc.description.affiliationDepartment of Pharmacological Sciences Icahn School of Medicine at Mount Sinai
dc.description.affiliationDepartamento de Ginecologia e Obstetrícia Faculdade de Medicina Universidade Estadual Paulista, SP
dc.description.affiliationDepartamento de Fisioterapia e Terapia Ocupacional Faculdade de Filosofia e Ciências Universidade Estadual Paulista, SP
dc.description.affiliationUnespDepartamento de Biotecnologia Faculdade de Ciências e Letras Universidade Estadual Paulista, SP
dc.description.affiliationUnespDepartamento de Ginecologia e Obstetrícia Faculdade de Medicina Universidade Estadual Paulista, SP
dc.description.affiliationUnespDepartamento de Fisioterapia e Terapia Ocupacional Faculdade de Filosofia e Ciências Universidade Estadual Paulista, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2014/03324-4
dc.description.sponsorshipIdCNPq: 27250
dc.identifierhttp://dx.doi.org/10.1590/1414-431X2022e12343
dc.identifier.citationBrazilian Journal of Medical and Biological Research, v. 55.
dc.identifier.doi10.1590/1414-431X2022e12343
dc.identifier.issn1414-431X
dc.identifier.scopus2-s2.0-85143278703
dc.identifier.urihttp://hdl.handle.net/11449/247978
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.sourceScopus
dc.subjectAdipose tissue
dc.subjectCollagenase
dc.subjectPorcine
dc.subjectProtocol
dc.subjectStem Cells
dc.titleIsolation and characterization of farm pig adipose tissue-derived mesenchymal stromal/stem cellsen
dc.typeArtigo

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Artigos - Ginecologia e Obstetrícia - FMB
Artigos - Fisioterapia e Terapia Ocupacional - FFC