MicroRNAs expression influence in ulcerative colitis and Crohn's disease: A pilot study for the identification of diagnostic biomarkers

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dc.contributor.authorQuaglio, Ana Elisa Valencise [UNESP]
dc.contributor.authorSantaella, Felipe Jose [UNESP]
dc.contributor.authorRodrigues, Maria Aparecida Marchesan [UNESP]
dc.contributor.authorSassaki, Ligia Yukie [UNESP]
dc.contributor.authorDi Stasi, Luiz Claudio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:38:02Z
dc.date.available2022-04-29T08:38:02Z
dc.date.issued2021-12-07
dc.description.abstractfrom the Pathology Department of Botucatu Medical School at São Paulo State University (Unesp). The diagnosis of UC or CD was based on clinical, endoscopic, radiologic, and histological criteria and confirmed by histopathological analysis at the time of selection. The TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 (Applied Biosystems™) platform was used to analyze 754 miRNAs. Targets of DE-miRNAs were predicted using miRNA Data Integration Portal (mirDIP) and the miRNA Target Interaction database (MiRTarBase). All statistical analyses were conducted using GraphPad Prism software. Parametric and nonparametric data were analyzed using t-tests and Mann-Whitney U tests, respectively. RESULTS The results showed that of the 754 miRNAs that were initially evaluated, 643 miRNAs were found to be expressed in at least five of the patients who were diagnosed with either CD or UC; the remaining 111 miRNAs were not considered to be expressed in these patients. The expression levels of 28 miRNAs were significantly different between the CD and UC patients (P ≤ 0.05); 13 miRNAs demonstrated a fold-change in expression level greater than 1. Five miRNAs with a downregulated expression were selected for enrichment analysis. The miRNAs whose expression levels were significantly lower in UC patients than in CD patients were enriched in certain signaling pathways that were mostly correlated with cancer-related processes and respective biomarkers. CONCLUSION MiRNAs could be used to differentiate UC from CD, and differently expressed miRNAs could help explain the distinct pathophysiology of each disease. BACKGROUND Inflammatory bowel disease (IBD) comprises two distinct diseases, Crohn’s disease (CD) and ulcerative colitis (UC), both of which are chronic, relapsing inflammatory disorders of the gastrointestinal tract with a mostly unknown etiology. The incidence and prevalence of IBD are continually increasing, indicating the need for further studies to investigate the genetic determinants of these diseases. Since microRNAs (miRNAs) regulate protein translation via complementary binding to mRNA, discovering differentially expressed miRNAs (DE) in UC or CD patients could be important for diagnostic biomarker identification, assisting in the appropriate disease differentiation progressing the understanding of IBD pathogenesis. AIM To determine the miRNA expression profile in UC and CD patients and the potential pathophysiological contributions of differentially expressed miRNA. METHODS A total of 20 formalin-fixed paraffin-embedded colonic samples were collecteden
dc.description.affiliationLaboratory of Phytomedicines Pharmacology and Biotechnology (PhytoPharmaTec) Department of Biophysics and Pharmacology São Paulo State University (Unesp) Institute of Biosciences Botucatu
dc.description.affiliationDepartment of Pathology Botucatu Medical School Sao Paulo State University (Unesp) Botucatu
dc.description.affiliationDepartment of Internal Medicine Botucatu Medical School São Paulo State University (Unesp) Botucatu
dc.description.affiliationUnespLaboratory of Phytomedicines Pharmacology and Biotechnology (PhytoPharmaTec) Department of Biophysics and Pharmacology São Paulo State University (Unesp) Institute of Biosciences Botucatu
dc.description.affiliationUnespDepartment of Pathology Botucatu Medical School Sao Paulo State University (Unesp) Botucatu
dc.description.affiliationUnespDepartment of Internal Medicine Botucatu Medical School São Paulo State University (Unesp) Botucatu
dc.format.extent7801-7812
dc.identifierhttp://dx.doi.org/10.3748/wjg.v27.i45.7801
dc.identifier.citationWorld Journal of Gastroenterology, v. 27, n. 45, p. 7801-7812, 2021.
dc.identifier.doi10.3748/wjg.v27.i45.7801
dc.identifier.issn2219-2840
dc.identifier.issn1007-9327
dc.identifier.scopus2-s2.0-85121817530
dc.identifier.urihttp://hdl.handle.net/11449/230120
dc.language.isoeng
dc.relation.ispartofWorld Journal of Gastroenterology
dc.sourceScopus
dc.subjectBiomarker
dc.subjectCrohn’s disease
dc.subjectDifferential diagnosis
dc.subjectInflammatory bowel disease
dc.subjectMiRNA
dc.subjectUlcerative colitis
dc.titleMicroRNAs expression influence in ulcerative colitis and Crohn's disease: A pilot study for the identification of diagnostic biomarkersen
dc.typeArtigo

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