Acquired pellicle protein-based engineering protects against erosive demineralization

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dc.contributor.authorCarvalho, Thamyris Souza
dc.contributor.authorAraújo, Tamara Teodoro
dc.contributor.authorVentura, Talita Mendes Oliveira
dc.contributor.authorDionizio, Aline
dc.contributor.authorCâmara, João Victor Frazão
dc.contributor.authorMoraes, Samanta Mascarenhas
dc.contributor.authorPelá, Vinicius Taioqui
dc.contributor.authorMartini, Tatiana
dc.contributor.authorLeme, Julia Chaparro
dc.contributor.authorDerbotolli, Ana Luiza Bogaz
dc.contributor.authorGrizzo, Larissa Tercilia
dc.contributor.authorCrusca, Edson [UNESP]
dc.contributor.authorShibao, Priscila Yumi Tanaka
dc.contributor.authorMarchetto, Reinaldo [UNESP]
dc.contributor.authorHenrique-Silva, Flavio
dc.contributor.authorPessan, Juliano Pelim [UNESP]
dc.contributor.authorBuzalaf, Marília Afonso Rabelo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSão Carlos Federal University
dc.date.accessioned2021-06-25T10:35:09Z
dc.date.available2021-06-25T10:35:09Z
dc.date.issued2020-11-01
dc.description.abstractObjectives: To evaluate, in vivo: 1) proteomic alterations in the acquired enamel pellicle (AEP) after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), statherin-derived peptide (StN15) or their combination before the formation of the AEP and subsequent erosive challenge; 2) the protection of these treatments against erosive demnineralization. Materials and methods: In 5 crossover phases, after prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with: deionized water-1, 0.1 mg/mL CaneCPI-5-2, 1.0 mg/mL HB-3, 1.88 × 10-5 M StN15-4 or their combination-5. AEP was formed (2 h) and enamel biopsy (10 μL, 1%citric acid, pH 2.5, 10 s) was performed on one incisor for calcium analysis. The same acid was applied on the vestibular surfaces of the remaining teeth. The acid-resistant proteins within the remaining AEP were collected. Samples were quantitatively analyzed by label-free proteomics. Results: Treatment with the proteins/peptide, isolated or combined, increased several acid-resistant proteins in the AEP, compared with control. The highest increases were seen for PRPs (32-fold, StN15), profilin (15-fold, combination), alpha-amylase (9-fold; StN15), keratins (8-fold, CaneCPI-5 and HB), Histatin-1 (7-fold, StN15), immunoglobulins (6.5-fold, StN15), lactotransferrin (4-fold, CaneCPI-5), cystatins, lysozyme, protein S-100-A9 and actins (3.5-fold, StN15), serum albumin (3.5-fold, CaneCPI-5 and HB) and hemoglobin (3-fold, StN15). Annexin, calmodulin, keratin, tubulin and cystatins were identified exclusively upon treatment with the proteins/peptide, alone or combined. Groups 2, 3 and 4 had significantly lower Ca released from enamel compared to group 1 (Kruskal-Wallis/Dunn's, p < 0.05). Conclusions: Treatment with CaneCPI-5, HB or StN15 remarkably increases acid-resistant proteins in the AEP, protecting against erosion. Clinical significance: Our results show, for the first time, that treatment with proteins/peptide remarkably increases acid-resistant proteins in the AEP, protecting against erosive demineralization. These findings open an avenue for a new preventive approach for erosive demineralization, employing acquired pellicle engineering procedures that may in the future be incorporated into dental products.en
dc.description.affiliationDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo
dc.description.affiliationDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Genetics and Evolution São Carlos Federal University
dc.description.affiliationDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2018/12041-7
dc.description.sponsorshipIdFAPESP: 2019/16254-8
dc.description.sponsorshipIdCNPq: 407853/2018-9
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2020.103478
dc.identifier.citationJournal of Dentistry, v. 102.
dc.identifier.doi10.1016/j.jdent.2020.103478
dc.identifier.issn0300-5712
dc.identifier.scopus2-s2.0-85092044048
dc.identifier.urihttp://hdl.handle.net/11449/206608
dc.language.isoeng
dc.relation.ispartofJournal of Dentistry
dc.sourceScopus
dc.subjectAcquired enamel pellicle
dc.subjectCystatin
dc.subjectDental erosion
dc.subjectHemoglobin
dc.subjectProteomics
dc.subjectStatherin
dc.titleAcquired pellicle protein-based engineering protects against erosive demineralizationen
dc.typeArtigo
unesp.author.orcid0000-0002-8628-9524[1]
unesp.author.orcid0000-0002-8687-0124[4]
unesp.author.orcid0000-0002-9687-4401[5]
unesp.author.orcid0000-0002-4256-1848[6]
unesp.author.orcid0000-0002-7519-049X[12]
unesp.author.orcid0000-0002-8757-321X[14]
unesp.author.orcid0000-0002-1550-3933[16]
unesp.author.orcid0000-0002-5985-3951[17]

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