PTEN Immunohistochemistry: A New Approach for Diagnosis of Intestinal Neuronal Dysplasia Type B

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dc.contributor.authorTerra, Simone Antunes [UNESP]
dc.contributor.authorde Arruda Lourenção, Pedro Luiz Toledo [UNESP]
dc.contributor.authorRodrigues, Maria Aparecida Marchesan [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:55:11Z
dc.date.available2023-07-29T13:55:11Z
dc.date.issued2023-05-01
dc.description.abstractContext.—Intestinal neuronal dysplasia type B (IND B) is a complex entity involving the enteric nervous system, clinically manifested with constipation in infancy. Diagnosis has been established by histopathologic analysis of rectal biopsies. However, the criteria for the diagnosis have been questioned and modified, hindering diagnostic practice. Objective.—To analyze the applicability of PTEN immunohistochemistry in the diagnosis of IND B and to compare with control cases and cases of Hirschsprung disease (HD). Design.—PTEN immunohistochemical expression was analyzed in colorectal samples from 29 cases of IND B and compared with 4 control cases and 6 cases of HD. The pattern of PTEN immunoexpression was analyzed in glial cells of the submucosal and myenteric nerve plexuses and in neural fibrils of the muscularis propria using a scoring system. Results.—Marked reduction or absence of PTEN expression was observed in glial cells of the submucosal nerve plexuses in all cases of the IND B group and in the myenteric nerve plexuses in 28 of 29 cases (96.5%). Lack of PTEN expression was detected in neural fibrils within the muscularis propria in 21 of 29 cases (72%) of the IND B group. PTEN expression was positive in the same neural structures of the control and HD groups. Conclusions.—PTEN immunohistochemistry may be a valuable tool in the diagnostic evaluation of IND B. Lack of or reduction of PTEN expression in neural fibrils within the muscularis propria suggests that involvement of the neuromuscular junction may be a key event in the pathogenesis of the motility disturbance occurring in IND B.en
dc.description.affiliationThe Departments of Pathology Botucatu Medical School Unesp–Sao Paulo State University, SP
dc.description.affiliationSurgery–Division of Pediatric Surgery Botucatu Medical School Unesp–Sao Paulo State University, SP
dc.description.affiliationUnespThe Departments of Pathology Botucatu Medical School Unesp–Sao Paulo State University, SP
dc.description.affiliationUnespSurgery–Division of Pediatric Surgery Botucatu Medical School Unesp–Sao Paulo State University, SP
dc.format.extent577-583
dc.identifierhttp://dx.doi.org/10.5858/arpa.2021-0424-OA
dc.identifier.citationArchives of Pathology and Laboratory Medicine, v. 147, n. 5, p. 577-583, 2023.
dc.identifier.doi10.5858/arpa.2021-0424-OA
dc.identifier.issn1543-2165
dc.identifier.issn0003-9985
dc.identifier.scopus2-s2.0-85159544153
dc.identifier.urihttp://hdl.handle.net/11449/248840
dc.language.isoeng
dc.relation.ispartofArchives of Pathology and Laboratory Medicine
dc.sourceScopus
dc.titlePTEN Immunohistochemistry: A New Approach for Diagnosis of Intestinal Neuronal Dysplasia Type Ben
dc.typeArtigo

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