Analytical approach to the metallomic of Nile tilapia (Oreochromis niloticus) liver tissue by SRXRF and FAAS after 2D-PAGE separation: Preliminary results

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dc.contributor.authorLima, Paula M. [UNESP]
dc.contributor.authorNeves, Renato de C. F. [UNESP]
dc.contributor.authordos Santos, Felipe A. [UNESP]
dc.contributor.authorPerez, Carlos A.
dc.contributor.authorda Silva, Marcelo O. A.
dc.contributor.authorArruda, Marco A. Z.
dc.contributor.authorde Castro, Gustavo R. [UNESP]
dc.contributor.authorPadilha, Pedro M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionLab Nacl Luz Sincronton
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T13:53:35Z
dc.date.available2014-05-20T13:53:35Z
dc.date.issued2010-08-15
dc.description.abstractAn investigation was made into calcium, iron and zinc in protein spots in samples of Nile tilapia (Oreochromis niloticus) liver tissue obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (20-PAGE) and subsequent qualitative and quantitative evaluation by synchrotron radiation X-ray fluorescence (SRXRF) and Flame Atomic Absorption Spectrometry (FAAS). An analysis of the fluorescence spectra indicated the presence of calcium, iron and zinc in 12, 6 and 8 liver protein spots, respectively. The metal ions found were distributed mainly in proteins with a molar mass of less than 40.00 kDa and more than 12.00 kDa, with pI in the range of 4.70-9.40. The only exception was a spot presenting protein with a molar mass of 10.10 kDa. In addition to calcium, iron and zinc, sulfur and phosphorus - which are non-metals that may be part of the protein structure, were also detected. After microwave-assisted acid mineralization of the proteins spots, a FAAS estimation of the concentration of calcium, iron and zinc ions bound to these proteins indicated a range of 1.08-5.80 mg g(-1), 2.02-8.03 mg g(-1) and 1.60-8.55 mg g(-1), respectively. (C) 2010 Published by Elsevier B.V.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Quim & Bioquim, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUNESP, Fac Med Vet & Zootecn, Programa Posgrad Zootecn, BR-15385000 Botucatu, SP, Brazil
dc.description.affiliationLab Nacl Luz Sincronton, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Quim, GEPAM, BR-13084971 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Nacl Ciência & Tecnol Bioanalit, BR-13084971 Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Quim & Bioquim, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med Vet & Zootecn, Programa Posgrad Zootecn, BR-15385000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 03/13362-6
dc.description.sponsorshipIdFAPESP: 07/59778-0
dc.description.sponsorshipIdCNPq: 301123/2005-5
dc.format.extent1052-1056
dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2010.06.023
dc.identifier.citationTalanta. Amsterdam: Elsevier B.V., v. 82, n. 3, p. 1052-1056, 2010.
dc.identifier.doi10.1016/j.talanta.2010.06.023
dc.identifier.issn0039-9140
dc.identifier.lattes6981448637456391
dc.identifier.orcid0000-0003-4179-0574
dc.identifier.urihttp://hdl.handle.net/11449/19127
dc.identifier.wosWOS:000281177100029
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.ispartofjcr4.244
dc.relation.ispartofsjr1,186
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectMetallomicsen
dc.subjectProtein spotsen
dc.subject2D-PAGEen
dc.subjectSRXRFen
dc.subjectFAASen
dc.titleAnalytical approach to the metallomic of Nile tilapia (Oreochromis niloticus) liver tissue by SRXRF and FAAS after 2D-PAGE separation: Preliminary resultsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes6981448637456391[8]
unesp.author.orcid0000-0003-4284-3148[4]
unesp.author.orcid0000-0003-4179-0574[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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