Maternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in rats

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dc.contributor.authorPinheiro, Daniela F. [UNESP]
dc.contributor.authorPinheiro, Patricia F.F. [UNESP]
dc.contributor.authorBuratini, José [UNESP]
dc.contributor.authorCastilho, Anthony C.S. [UNESP]
dc.contributor.authorLima, Paula F. [UNESP]
dc.contributor.authorTrinca, Luiza A. [UNESP]
dc.contributor.authorVicentini-Paulino, Maria de Lourdes M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:33Z
dc.date.available2014-05-27T11:30:33Z
dc.date.issued2013-09-01
dc.description.abstractIntrauterine dietary restriction may cause changes in the functioning of offspring organs and systems later in life, an effect known as fetal programming. The present study evaluated mRNA abundance and immunolocalization of nutrient transporters as well as enterocytes proliferation in the proximal, median and distal segments of small intestine of rats born to protein-restricted dams. Pregnant rats were fed hypoproteic (6% protein) or control (17% protein) diets, and offspring rats were evaluated at 3 and 16 weeks of age. The presence of SGLT1 (sodium-glucose co-transporter 1), GLUT2 (glucose transporter 2), PEPT1 (peptide transporter 1) and the intestinal proliferation were evaluated by immunohistochemical techniques and the abundance of specific mRNA for SGLT1, GLUT2 and PEPT1 was assessed by the real-time PCR technique. Rats born to protein-restricted dams showed higher cell proliferation in all intestinal segments and higher gene expression of SGLT1 and PEPT1 in the duodenum. Moreover, in adult animals born to protein-restricted dams the immunoreactivity of SGLT1, GLUT2 and PEPT1in the duodenum was more intense than in control rats. Taken together, the results indicate that changes in the small intestine observed in adulthood can be programmed during the gestation. In addition, they show that this response is caused by both up-regulation in transporter gene expression, a specific adaptation mechanism, and intestinal proliferation, an unspecific adaptation mechanism.en
dc.description.affiliationDepartment of Physiology Universidade Estadual Paulista (UNESP), Botucatu
dc.description.affiliationDepartment of Anatomy Universidade Estadual Paulista (UNESP), Botucatu
dc.description.affiliationDepartment of Statistics-Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu
dc.description.affiliationUnespDepartment of Physiology Universidade Estadual Paulista (UNESP), Botucatu
dc.description.affiliationUnespDepartment of Anatomy Universidade Estadual Paulista (UNESP), Botucatu
dc.description.affiliationUnespDepartment of Statistics-Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu
dc.format.extent281-289
dc.identifierhttp://dx.doi.org/10.1042/CS20120400
dc.identifier.citationClinical Science, v. 125, n. 6, p. 281-289, 2013.
dc.identifier.doi10.1042/CS20120400
dc.identifier.issn0143-5221
dc.identifier.lattes5760560970751598
dc.identifier.orcid0000-0003-1452-5708
dc.identifier.scopus2-s2.0-84878657380
dc.identifier.urihttp://hdl.handle.net/11449/76429
dc.identifier.wosWOS:000322504400006
dc.language.isoeng
dc.relation.ispartofClinical Science
dc.relation.ispartofjcr5.220
dc.relation.ispartofsjr2,166
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectFetal programming
dc.subjectIntestinal transporter
dc.subjectPeptide transporter 1 (PEPT1)
dc.subjectSodium-glucose co-transporter 1 (SGLT1)
dc.subjectglucose transporter 2
dc.subjectmessenger RNA
dc.subjectpeptide transporter 1
dc.subjectsodium glucose cotransporter 2
dc.subjectadult animal
dc.subjectanimal tissue
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectduodenum
dc.subjectfemale
dc.subjectgene expression
dc.subjectimmunolocalization
dc.subjectimmunoreactivity
dc.subjectintestine cell
dc.subjectmale
dc.subjectmaternal nutrition
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprogeny
dc.subjectprotein restriction
dc.subjectrat
dc.subjectreal time polymerase chain reaction
dc.subjectsmall intestine
dc.subjectAdaptation, Physiological
dc.subjectAdiposity
dc.subjectAnimal Nutritional Physiological Phenomena
dc.subjectAnimals
dc.subjectBody Weight
dc.subjectCell Proliferation
dc.subjectDiet, Protein-Restricted
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectGene Expression Regulation
dc.subjectGlucose Transporter Type 2
dc.subjectImmunohistochemistry
dc.subjectIntestine, Small
dc.subjectMalnutrition
dc.subjectMaternal Nutritional Physiological Phenomena
dc.subjectMembrane Transport Proteins
dc.subjectPregnancy
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectSodium-Glucose Transporter 1
dc.subjectSymporters
dc.titleMaternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in ratsen
dc.typeArtigo
dcterms.licensehttp://www.portlandpress.com/pp/journals/rights.htm
unesp.author.lattes5760560970751598[2]
unesp.author.orcid0000-0003-1106-8505[6]
unesp.author.orcid0000-0003-1452-5708[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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