Influence of culture conditions on the secretome of mesenchymal stem cells derived from feline adipose tissue: Proteomics approach

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dc.contributor.authorLara, Maria Laura [UNESP]
dc.contributor.authorCarvalho, Marcos Gomides [UNESP]
dc.contributor.authorde Souza, Fabiana Ferreira [UNESP]
dc.contributor.authorSchmith, Rubia Alves [UNESP]
dc.contributor.authorCodognoto, Viviane Maria [UNESP]
dc.contributor.authorDe Vita, Bruna [UNESP]
dc.contributor.authorFreitas Dell'Aqua, Camila de Paula [UNESP]
dc.contributor.authorLandim, Fernada da Cruz [UNESP]
dc.contributor.authorAlvarenga, Marina Landim e [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversitat de Barcelona
dc.contributor.institutionOmics Animal Biotechnology
dc.date.accessioned2023-07-29T13:46:24Z
dc.date.available2023-07-29T13:46:24Z
dc.date.issued2023-08-01
dc.description.abstractThis study aimed to describe the secretome of mesenchymal stem cells derived from feline adipose tissue (AD-MSCs) and compare the effects of different culture conditions on AD-MSC proteomics using a shotgun approach. Adipose tissue was collected from 5 female cats and prepared to culture. Conditioned media was collected at third passage, in which the cells were cultured under 4 conditions, normoxia with fetal bovine serum (N + FBS), hypoxia with FBS (H + FBS), normoxia without FBS (N – FBS), and hypoxia without FBS (H – FBS). Then, the secretome was concentrated and prepared for proteomic approaches. Secretomes cultured with FBS-free medium had more than twice identified proteins in comparison with the secretomes cultured with FBS. In contrast, hypoxic conditions did not increase protein amount and affected only a small proteome fraction. Relevant proteins were related to the extracellular matrix promoting environmental modulation, influencing cell signaling pathways, and providing a suitable environment for cell proliferation and maintenance. Moreover, other proteins were also related to cell adhesion, migration and morphogenesis. Culture conditions can influence protein abundance in AD-MSC secretome, and can give also more specificity to cell and cell-free treatments for different diseases.en
dc.description.affiliationDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University - UNESP, Botucatu
dc.description.affiliationInternational Product Marketing Manager - PROCARE HEALTH Universitat de Barcelona, Catalunha
dc.description.affiliationOmics Animal Biotechnology, Botucatu
dc.description.affiliationUnespDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University - UNESP, Botucatu
dc.format.extent78-86
dc.identifierhttp://dx.doi.org/10.1016/j.biochi.2023.03.004
dc.identifier.citationBiochimie, v. 211, p. 78-86.
dc.identifier.doi10.1016/j.biochi.2023.03.004
dc.identifier.issn6183-1638
dc.identifier.issn0300-9084
dc.identifier.scopus2-s2.0-85150336368
dc.identifier.urihttp://hdl.handle.net/11449/248527
dc.language.isoeng
dc.relation.ispartofBiochimie
dc.sourceScopus
dc.subjectConditioned medium
dc.subjectFelis catus
dc.subjectHypoxia-culture
dc.subjectProteomics
dc.subjectSerum-free-culture
dc.subjectStem cell
dc.titleInfluence of culture conditions on the secretome of mesenchymal stem cells derived from feline adipose tissue: Proteomics approachen
dc.typeArtigo
unesp.author.orcid0000-0002-1080-4163[1]

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