Contractile effects of serotonin (5-HT) in the rat cauda epididymis: Expression and functional characterization of 5-HT receptors

Página do item simplificado
dc.contributor.authorMueller, Andre
dc.contributor.authorKiguti, Luiz R.A. [UNESP]
dc.contributor.authorSilva, Erick J.R. [UNESP]
dc.contributor.authorPupo, André S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2019-10-06T16:20:44Z
dc.date.available2019-10-06T16:20:44Z
dc.date.issued2019-04-01
dc.description.abstractSerotonin [5-hydroxytryptamine (5-HT)] exerts multiple central and peripheral functions. High concentrations of 5-HT have been found in the epididymis, a ductal organ that plays pivotal roles in sperm transport and maturation. The contraction of the epididymal smooth muscle is essential for sperm transport and emission during ejaculation. The contributions of the epididymal 5-HT system to these events are poorly understood. Here, we assessed the contractile function of 5-HT in the rat cauda epididymis (CE), pharmacologically targeting the receptor(s) and the reuptake mechanism involved in this system. Segments of CE duct from adult Wistar rats were set up in an organ bath system for isometric tension recordings, and concentration-response curves to 5-HT and norepinephrine were obtained. 5-HT elicited concentration-dependent contractions of the CE duct (pEC50 5 6.5 6 0.1) that were potentiated with high potency by the norepinephrine transporter (NET) inhibitor desipramine and with low potency by the highly selective serotonin transporter inhibitor paroxetine, indicating that the NET is the major mediator of 5-HT reuptake in vitro. CE contractions to 5-HT were antagonized by the a1-adrenoceptor (a1-AR) antagonist prazosin (pA2 @ 8.9), 5-HT2A/2C antagonists ketanserin (pA2 @ 9.4) and fluoxetine (pA2 @ 7.4), and 5-HT1A ligands WAY 100635 (pA2 @ 8.9) and buspirone (pA2 @ 7.3). Reverse transcriptase polymerase chain reaction analysis demonstrated that 5-HT1A and 5-HT2A transcripts are highly abundant in the cauda epididymis, whereas 5-HT2C transcript was not found. Altogether, our results reveal that contractions of the CE duct to 5-HT encompasses at least activation of a1-ARs and 5-HT1A and 5-HT2A receptors, providing new insights into the roles of 5-HT on the epididymal function.en
dc.description.affiliationDepartment of Pharmacology Institute of Biosciences São Paulo State University
dc.description.affiliationDepartment of Pharmacology Universidade Estadual de Campinas
dc.description.affiliationDepartment of Pharmacology Institute of Biosciences UNESP - São Paulo State University, Professor Dr. Antonio Celso W. Zanin Street, Rubião Junior District
dc.description.affiliationUnespDepartment of Pharmacology Institute of Biosciences São Paulo State University
dc.description.affiliationUnespDepartment of Pharmacology Institute of Biosciences UNESP - São Paulo State University, Professor Dr. Antonio Celso W. Zanin Street, Rubião Junior District
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 15175-1
dc.description.sponsorshipIdFAPESP: 2015/08227-0
dc.format.extent98-106
dc.identifierhttp://dx.doi.org/10.1124/jpet.118.254110
dc.identifier.citationJournal of Pharmacology and Experimental Therapeutics, v. 369, n. 1, p. 98-106, 2019.
dc.identifier.doi10.1124/jpet.118.254110
dc.identifier.issn1521-0103
dc.identifier.issn0022-3565
dc.identifier.scopus2-s2.0-85062837672
dc.identifier.urihttp://hdl.handle.net/11449/188834
dc.language.isoeng
dc.relation.ispartofJournal of Pharmacology and Experimental Therapeutics
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleContractile effects of serotonin (5-HT) in the rat cauda epididymis: Expression and functional characterization of 5-HT receptorsen
dc.typeArtigo
unesp.author.lattes2224433126054725[4]
unesp.author.orcid0000-0001-6627-3448[4]

Arquivos

Coleções

Artigos - Farmacologia - IBB