The effect of changes in the bulk dielectric constant on the DNA torsional properties

Página do item simplificado
dc.contributor.authorSouza, Fátima Pereira de
dc.contributor.authorAgostinho Neto, Augusto
dc.contributor.authorFossey, Marcelo Andrés [UNESP]
dc.contributor.authorRuggiero Neto, Joao
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:00:24Z
dc.date.available2014-05-20T14:00:24Z
dc.date.issued2007-11-01
dc.description.abstractThe effect of changes in the bulk dielectric constant on the DNA torsional properties was evaluated from plasmid circularization reactions. In these reactions, pUC18 previously linearized by EcoRI digestion was recircularized with T4 DNA ligase. The bulk dielectric constant of the reaction medium was decreased by the addition of different concentrations of neutral solutes: ethylene glycol, glycerol, sorbitol, and sucrose, or increased by the addition of glycine. The topoisomers generated by the ligase reaction were resolved by agarose-gel electrophoresis. The DNA twist energy parameter (K), which is an apparent torsional constant, was determined by linearization of the Gaussian topoisomers' distribution. It was observed that the twist energy parameter for the given solutes is almost linearly dependent on the bulk dielectric constant. In the reaction buffer, the twist energy parameter was determined to be 1100 +/- 100. By decreasing the dielectric constant to 74 with the addition of sorbitol, the value of the parameter reaches K = 900 +/- 100, whereas the addition of ethylene glycol leads to kappa = 400 +/- 50. Upon addition of glycine, which resulted in a dielectric constant equal to 91, the value of the twist energy parameter increased to K 1750 +/- 100. (c) 2007 Wiley Periodicals.en
dc.description.affiliationUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Biol, IBILCE, Sao Jose Dos Campos, Brazil
dc.description.affiliationUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Fis, IBILCE, Sao Jose Dos Campos, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Biol, IBILCE, Sao Jose Dos Campos, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Fis, IBILCE, Sao Jose Dos Campos, Brazil
dc.format.extent244-248
dc.identifierhttp://dx.doi.org/10.1002/bip.20830
dc.identifier.citationBiopolymers. Hoboken: John Wiley & Sons Inc., v. 87, n. 4, p. 244-248, 2007.
dc.identifier.doi10.1002/bip.20830
dc.identifier.issn0006-3525
dc.identifier.lattes4101562077663619
dc.identifier.lattes3313511334783986
dc.identifier.orcid0000-0002-4731-4977
dc.identifier.urihttp://hdl.handle.net/11449/21366
dc.identifier.wosWOS:000250210700005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofBiopolymers
dc.relation.ispartofjcr1.990
dc.relation.ispartofsjr0,861
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDNApt
dc.subjectdielectric constantpt
dc.subjectelectrophoresispt
dc.subjecttorsional energetic parameterpt
dc.subjecthelical twist anglept
dc.subjectneutral solutespt
dc.titleThe effect of changes in the bulk dielectric constant on the DNA torsional propertiesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes4101562077663619
unesp.author.lattes3313511334783986[1]
unesp.author.orcid0000-0002-2012-388X[3]
unesp.author.orcid0000-0002-4731-4977[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Biologia - IBILCE
Artigos - Física - IBILCE