Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms

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dc.contributor.authorMouta, Luís Felipe Garcia Leal [UNESP]
dc.contributor.authorMarques, Raquel Souza [UNESP]
dc.contributor.authorKoga-Ito, Cristiane Yumi [UNESP]
dc.contributor.authorSalvador, Marcos José
dc.contributor.authorGiro, Elisa Maria Aparecida [UNESP]
dc.contributor.authorBrighenti, Fernanda Lourenção [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2023-07-29T13:26:22Z
dc.date.available2023-07-29T13:26:22Z
dc.date.issued2022-10-01
dc.description.abstractThe aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.5% sucrose exposure for 6 h/day) for 3 days in 24-well plates. The biofilms were treated twice a day and their composition was evaluated by microorganism quantification. The acidogenicity was evaluated by measuring the pH of the spent culture medium in contact with the biofilm. Overall, the association of C. citratus and chlorhexidine reduced total bacterial counts and aciduric bacteria (maximum reduction of 3.55 log UFC/mL) in microcosm biofilms. This group also presented the lowest acidogenicity even when exposed to sucrose-containing medium. C. citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms. Based on these findings, this study can contribute to the development of new formulations that might allow for the use of mouthwashes for a shorter period, which may reduce undesirable effects and increase patient compliance to the treatment.en
dc.description.affiliationDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry Araraquara Dental School São Paulo State University—UNESP, R Humaitá, 1680
dc.description.affiliationDepartment of Environment Engineering Institute of Science and Technology São José dos Campos São Paulo State University—UNESP, Av Eng Francisco José Longo, 777
dc.description.affiliationDepartment of Plant Biology Institute of Biology University of Campinas—UNICAMP, R Carlos Gomes, 241
dc.description.affiliationUnespDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry Araraquara Dental School São Paulo State University—UNESP, R Humaitá, 1680
dc.description.affiliationUnespDepartment of Environment Engineering Institute of Science and Technology São José dos Campos São Paulo State University—UNESP, Av Eng Francisco José Longo, 777
dc.identifierhttp://dx.doi.org/10.3390/pathogens11101067
dc.identifier.citationPathogens, v. 11, n. 10, 2022.
dc.identifier.doi10.3390/pathogens11101067
dc.identifier.issn2076-0817
dc.identifier.scopus2-s2.0-85140833838
dc.identifier.urihttp://hdl.handle.net/11449/247808
dc.language.isoeng
dc.relation.ispartofPathogens
dc.sourceScopus
dc.subjectbiofilm
dc.subjectbiological products
dc.subjectdental caries
dc.subjectessential oils
dc.subjectphytotherapy
dc.titleCymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilmsen
dc.typeArtigo
unesp.author.orcid0000-0002-2416-2173[3]
unesp.author.orcid0000-0002-3839-5445[4]
unesp.author.orcid0000-0002-4470-5171[6]

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