Determination of amino acids in gym supplements using digital images and paper platform coupled to diffuse reflectance spectroscopy and USB device

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dc.contributor.authorCatelani, Tiago Augusto [UNESP]
dc.contributor.authorBittar, Dayana Borges [UNESP]
dc.contributor.authorPezza, Leonardo [UNESP]
dc.contributor.authorPezza, Helena Redigolo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:59:10Z
dc.date.available2019-10-06T16:59:10Z
dc.date.issued2019-05-01
dc.description.abstractNew and reliable methodologies are described for the determination of amino acids in gym supplements, offering rapid and clean analysis, with low generation of waste. The proposed methods are based on the reaction between ninhydrin and amino acids in acetate buffer medium (pH 4.75). Experimental design tools were used to optimize the analytical conditions. The linear range obtained for both methodologies was 20.0–350.0 mg L−1. The detection (LOD) and quantification (LOQ) limits were 6.2 and 21.0 mg L−1, respectively, for diffuse reflectance using a paper filter with hydrophobic barriers to increase the sensitivity and homogeneity of the colored product, and 5.7 and 18.8 mg L−1, respectively, for digital image analysis. A USB device was used in the diffuse reflectance spectroscopy method for heating the filter paper, providing speed and portability for the developed methodology. The methods showed good results when applied to gym supplement samples, with recoveries in the range 93.1–110%.en
dc.description.affiliationInstituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho” UNESP, R. Prof. Francisco Degni 55, P.O. Box 355
dc.description.affiliationUnespInstituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho” UNESP, R. Prof. Francisco Degni 55, P.O. Box 355
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/21733-1
dc.format.extent523-529
dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2018.12.052
dc.identifier.citationTalanta, v. 196, p. 523-529.
dc.identifier.doi10.1016/j.talanta.2018.12.052
dc.identifier.issn0039-9140
dc.identifier.lattes5978908591853524
dc.identifier.scopus2-s2.0-85059447801
dc.identifier.urihttp://hdl.handle.net/11449/190002
dc.language.isoeng
dc.relation.ispartofTalanta
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAmino acids
dc.subjectDiffuse reflectance spectroscopy
dc.subjectDigital image analysis
dc.subjectGym supplements
dc.subjectHydrophobic barrier
dc.subjectUSB device
dc.titleDetermination of amino acids in gym supplements using digital images and paper platform coupled to diffuse reflectance spectroscopy and USB deviceen
dc.typeArtigo
unesp.author.lattes5978908591853524

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Artigos - Química Orgânica - IQ
Artigos - Química Analítica - IQ