Esterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae)

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dc.contributor.authorBaffi, Milla Alves [UNESP]
dc.contributor.authorPereira, Cícero Donizete
dc.contributor.authorSouza, Guilherme Rocha Lino de
dc.contributor.authorBonetti, Ana Maria
dc.contributor.authorCeron, Carlos Roberto [UNESP]
dc.contributor.authorGourlart, Luiz Ricardo
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:03:03Z
dc.date.available2014-05-20T14:03:03Z
dc.date.issued2005-12-01
dc.description.abstractThe cattle tick Boophilus microplus causes great damage in livestock and is considered one of the most important tropical ectoparasites. The traditional method of control is based on the intensive use of pesticides, however the indiscriminate use of these compounds over the years has led to the selection of resistant ticks. Hydrolases, particularly esterases (EST), have been reported to be associated with acaricide resistance in B. microplus. We compared the esterase profile of susceptible and cypermethrin-resistant strains of adult B. microplus and a pyrethroid susceptible reference strain (the Mozzo strain) using polyacrylamide gel electrophoresis and specific staining. The electrophoretic profiles of protein extracts revealed the presence of four regions with esterase activity in the cypermethrin-resistant strain and three of these regions in the susceptible strains. The bands were numbered EST-1 to EST-4 in sequence (starting from the anode) according to their decrease in negative charge. The EST-1A and EST-1B enzymes were detected only in the resistant strain. The inhibition studies with eserine sulfate, copper sulfate, p- p-chloromercuribenzoate (pCMB), malathion and phenylmethylsulfonyl fluoride (PMSF) indicated that the EST-1A and EST-1B enzymes belong to the acetylcholinesterase class and are probably associated with resistance to acaricides in this Brazilian resistant strain of B. microplus.en
dc.description.affiliationUniversidade Federal de Uberlândia Instituto de Genética e Bioquímica Laboratório de Genética
dc.description.affiliationUniversidade Estadual Paulista Departamento de Química e Ciências Ambientais Laboratório de Genética e Bioquímica
dc.description.affiliationUnespUniversidade Estadual Paulista Departamento de Química e Ciências Ambientais Laboratório de Genética e Bioquímica
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent749-753
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572005000500016
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 28, n. 4, p. 749-753, 2005.
dc.identifier.doi10.1590/S1415-47572005000500016
dc.identifier.fileS1415-47572005000500016.pdf
dc.identifier.issn1415-4757
dc.identifier.lattes2307201085881971
dc.identifier.scieloS1415-47572005000500016
dc.identifier.urihttp://hdl.handle.net/11449/22215
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectBoophilus microplusen
dc.subjectPesticideen
dc.subjectResistanceen
dc.subjectEsteraseen
dc.titleEsterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae)en
dc.typeArtigo
unesp.author.lattes2307201085881971
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Química e Ciências Ambientais - IBILCE