Obesity Modifies the Proteomic Profile of the Periodontal Ligament

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dc.contributor.authorNogueira, Andressa V. B. [UNESP]
dc.contributor.authorLopes, Maria Eduarda S. [UNESP]
dc.contributor.authorMarcantonio, Camila C. [UNESP]
dc.contributor.authorSalmon, Cristiane R.
dc.contributor.authorMofatto, Luciana S.
dc.contributor.authorDeschner, James
dc.contributor.authorNociti-Junior, Francisco H.
dc.contributor.authorCirelli, Joni A. [UNESP]
dc.contributor.institutionUniversity Medical Center of the Johannes Gutenberg University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionSão Leopoldo Mandic Research Center
dc.date.accessioned2023-07-29T13:38:35Z
dc.date.available2023-07-29T13:38:35Z
dc.date.issued2023-01-01
dc.description.abstractThis study aimed to assess the obesity effects on the proteomic profile of the periodontal ligament of rats submitted to obesity induction by a high-fat diet. Eight Holtzman rats were divided into control (n = 3) and obese (n = 5) groups. The maxillae were histologically processed for laser capture microdissection of the periodontal ligament of the first maxillary molars. Peptide mixtures were analyzed by LC-MS/MS. A total of 1379 proteins were identified in all groups. Among them, 335 (24.30%) were exclusively detected in the obese group, while 129 (9.35%) proteins were uniquely found in the control group. Out of the 110 (7.98%) differentially abundant proteins, 10 were more abundant and 100 had decreased abundance in the obese group. A gene ontology analysis showed some proteins related to obesity in the “extracellular exosome” term among differentially identified proteins in the gene ontology cellular component terms Prelp, Sec13, and Sod2. These three proteins were upregulated in the obese group (p < 0.05), as shown by proteomic and immunohistochemistry analyses. In summary, our study presents novel evidence that the proteomic profile of the periodontal ligament is altered in experimental obesity induction, providing a list of differentially abundant proteins associated with obesity, which indicates that the periodontal ligament is responsive to obesity.en
dc.description.affiliationDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University
dc.description.affiliationDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University—UNESP, São Paulo
dc.description.affiliationDepartment of Prosthodontics and Periodontics Division of Periodontics Piracicaba Dental School University of Campinas—UNICAMP, São Paulo
dc.description.affiliationDepartment of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas—UNICAMP, São Paulo
dc.description.affiliationSão Leopoldo Mandic Research Center, São Paulo
dc.description.affiliationUnespDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University—UNESP, São Paulo
dc.identifierhttp://dx.doi.org/10.3390/ijms24021003
dc.identifier.citationInternational Journal of Molecular Sciences, v. 24, n. 2, 2023.
dc.identifier.doi10.3390/ijms24021003
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85146705354
dc.identifier.urihttp://hdl.handle.net/11449/248245
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectobesity
dc.subjectperiodontal ligament
dc.subjectprolargin
dc.subjectprotein Sec13 homolog
dc.subjectproteomics
dc.subjectsuperoxide dismutase
dc.titleObesity Modifies the Proteomic Profile of the Periodontal Ligamenten
dc.typeArtigo
unesp.author.orcid0000-0002-2756-5947[1]
unesp.author.orcid0000-0002-0610-7074[3]
unesp.author.orcid0000-0003-3304-5064[4]
unesp.author.orcid0000-0002-2087-7251[5]
unesp.author.orcid0000-0001-6809-8866[7]
unesp.author.orcid0000-0002-7082-9290[8]

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