A simple reversed phase high-performance liquid chromatography (HPLC) method for determination of in situ gelling curcumin-loaded liquid crystals in in vitro performance tests

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dc.contributor.authorFonseca-Santos, Bruno [UNESP]
dc.contributor.authorGremião, Maria Palmira Daflon [UNESP]
dc.contributor.authorChorilli, Marlus [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:00:52Z
dc.date.available2018-12-11T17:00:52Z
dc.date.issued2017-11-01
dc.description.abstractA simple, rapid, and sensitive analytical procedure has been developed and validated for the in vitro measurement of curcumin in samples from mucosae retention studies. Curcumin was analyzed by HPLC using a C18 column with UV detection at 425 nm. The mobile phase was acetonitrile and water (50:50 v/v) acidified with 2% acetic acid at a flow rate of 1.2 mL min−1. The curve range was linear for the receptor solution concentration range 0.5–75 μg mL−1. The specificity showed no interference with the biological matrix and excipients of the acceptor media. Intra and inter-day accuracy, and precision values were lower than 5% and were not statically different (P < 0.05). Recoveries ranged from 99.7% to 108%. The limits of detection and quantitation were 11.61 and 500 ng mL−1, respectively. The method is adequate to assay curcumin from esophageal porcine samples, enabling the determination of penetration profiles for in situ gelling curcumin-loaded liquid crystals by in vitro studies, and fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of curcumin in porcine esophageal mucosae.en
dc.description.affiliationSchool of Pharmaceutical Sciences UNESP – São Paulo State University Campus Araraquara Department of Drugs and Medicines
dc.description.affiliationUnespSchool of Pharmaceutical Sciences UNESP – São Paulo State University Campus Araraquara Department of Drugs and Medicines
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/03746-3
dc.format.extent1029-1037
dc.identifierhttp://dx.doi.org/10.1016/j.arabjc.2016.01.014
dc.identifier.citationArabian Journal of Chemistry, v. 10, n. 7, p. 1029-1037, 2017.
dc.identifier.doi10.1016/j.arabjc.2016.01.014
dc.identifier.issn1878-5352
dc.identifier.lattes9129780536724256
dc.identifier.lattes1427125996716282
dc.identifier.scopus2-s2.0-84958214233
dc.identifier.urihttp://hdl.handle.net/11449/172543
dc.language.isoeng
dc.relation.ispartofArabian Journal of Chemistry
dc.relation.ispartofsjr0,591
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCurcumin
dc.subjectHigh-performance liquid chromatography
dc.subjectLiquid crystals
dc.subjectPerformance tests
dc.subjectValidation
dc.titleA simple reversed phase high-performance liquid chromatography (HPLC) method for determination of in situ gelling curcumin-loaded liquid crystals in in vitro performance testsen
dc.typeArtigo
unesp.author.lattes9129780536724256
unesp.author.lattes1427125996716282

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