Dissolved air flotation as potential new mechanism for intestinal parasite diagnosis in feces

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dc.contributor.authorSoares, Felipe Augusto
dc.contributor.authorMartins dos Santos, Bianca
dc.contributor.authorRosa, Stefany Laryssa
dc.contributor.authorLoiola, Saulo Hudson Nery
dc.contributor.authorSuzuki, Celso Tetsuo Nagase
dc.contributor.authorSabadini, Edvaldo
dc.contributor.authorBresciani, Katia Denise Saraiva [UNESP]
dc.contributor.authorFalcão, Alexandre Xavier
dc.contributor.authorGomes, Jancarlo Ferreira
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionImmunoCamp Science and Technology
dc.date.accessioned2022-05-01T09:30:55Z
dc.date.available2022-05-01T09:30:55Z
dc.date.issued2021-12-01
dc.description.abstractThe parasitological examination of feces is recommended for the laboratory diagnosis of intestinal parasites due to its practicality, low-cost, and moderate diagnostic sensitivity. Dissolved Air Flotation (DAF) is an efficient technical principle used in other areas to separate dispersed solids. This study sought the preliminary evaluation of a proof-of-concept prototype as a tool for detecting species of parasites by adjusting DAF. Two DAF prototype units were developed to evaluate microbubbles' generation amidst fecal suspension and parasites' capture. For this evaluation, samples were screened and processed by the TF-Test technique (Control) and simultaneously by DAF device. The dimensional and attachment characteristics in the formation of the microbubbles were evaluated, and the percentage of parasitic recovery in floated and not-floated regions compared by Student's t-test. The second prototype unit proved to be more efficient in forming microbubbles with diameters between 34 and 170µm. The flotation tests showed a recovery of 73.27%, 58.12%, 37.85%, and 91.89% for Ascaris lumbricoides, Hymenolepis diminuta, Giardia duodenalis, and Strongyloides stercoralis, respectively. This study confirmed the selective interaction between microbubbles and parasite eggs and larvae during the flotation process using the DAF principle for the first time through imaging.en
dc.description.affiliationSchool of Medical Sciences University of Campinas
dc.description.affiliationLaboratory of Image Data Science (LIDS) Institute of Computing (IC) University of Campinas
dc.description.affiliationDepartment of Physical-Chemistry Institute of Chemistry University of Campinas
dc.description.affiliationSchool of Veterinary Medicine São Paulo State University
dc.description.affiliationImmunoCamp Science and Technology
dc.description.affiliationUnespSchool of Veterinary Medicine São Paulo State University
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2021.106137
dc.identifier.citationActa Tropica, v. 224.
dc.identifier.doi10.1016/j.actatropica.2021.106137
dc.identifier.issn1873-6254
dc.identifier.issn0001-706X
dc.identifier.scopus2-s2.0-85115936969
dc.identifier.urihttp://hdl.handle.net/11449/233602
dc.language.isoeng
dc.relation.ispartofActa Tropica
dc.sourceScopus
dc.subjectDissolved air flotation
dc.subjectParasitic intestinal diseases
dc.subjectParasitology
dc.subjectRoutine diagnostic tests
dc.titleDissolved air flotation as potential new mechanism for intestinal parasite diagnosis in fecesen
dc.typeArtigo
unesp.author.orcid0000-0001-6262-5194 0000-0001-6262-5194[1]
unesp.author.orcid0000-0003-4253-6631 0000-0003-4253-6631[3]

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