Photodithazine-mediated antimicrobial photodynamic therapy against fluconazole-resistant Candida albicans in vivo

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dc.contributor.authorAlves, Fernanda [UNESP]
dc.contributor.authorCarmello, Juliana Cabrini [UNESP]
dc.contributor.authorOliveira Mima, Ewerton Garcia de [UNESP]
dc.contributor.authorSouza Costa, Carlos Alberto de [UNESP]
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorPavarina, Ana Claudia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-04T12:40:55Z
dc.date.available2019-10-04T12:40:55Z
dc.date.issued2019-07-01
dc.description.abstractThis study evaluated the efficacy of antimicrobial Photodynamic Therapy (aPDT) against fluconazole-resistant Candida albicans in a murine model of oral candidosis. Mice were inoculated with two clinical isolates (R10, R15) and one reference strain (ATCC) of resistant C. albicans to produce oral candidosis. After inoculation, aPDT mediated by Photodithazine((R)) (PDZ) and LED light was performed. The use of PDZ or light only was also investigated. Additional animals were treated with Nystatin (NYS). Untreated or healthy mice were also evaluated. Microbiological evaluation was performed by recovering C. albicans from the tongue via colony-forming units. Animals were killed 24 hours after treatments, and the tongues were removed for histological analysis. Data were analyzed by one-way ANOVA and Tukey test (P < .05). The results demonstrated that all strains showed the same behavior after aPDT and NYS treatment. A significant reduction in C. albicans viability was achieved after both treatments for R15 and ATCC. No significant reduction was verified for C. albicans R10 submitted to aPDT or NYS. The histological analysis revealed that aPDT did not cause side effects on tissues. aPDT was effective for inactivation of two fluconazole-resistant C. albicans of the three strains evaluated.en
dc.description.affiliationSao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP, Brazil
dc.description.affiliationSao Paulo State Univ Unesp, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Phys Inst Sao Carlos, Grp Opt, Biophoton Lab, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ Unesp, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian Research Council
dc.description.sponsorshipIdFAPESP: 2011/15304-0
dc.description.sponsorshipIdFAPESP: 2012/02942-0
dc.description.sponsorshipIdFAPESP: 13/07276-1
dc.description.sponsorshipIdBrazilian Research Council: 304133/2016-7
dc.format.extent609-617
dc.identifierhttp://dx.doi.org/10.1093/mmy/myy083
dc.identifier.citationMedical Mycology. Oxford: Oxford Univ Press, v. 57, n. 5, p. 609-617, 2019.
dc.identifier.doi10.1093/mmy/myy083
dc.identifier.issn1369-3786
dc.identifier.urihttp://hdl.handle.net/11449/186068
dc.identifier.wosWOS:000482422300013
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofMedical Mycology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectphotochemotherapy
dc.subjectdrug resistance
dc.subjectoral candidiasis
dc.subjectCandida albicans
dc.titlePhotodithazine-mediated antimicrobial photodynamic therapy against fluconazole-resistant Candida albicans in vivoen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press
unesp.author.lattes8867670539105403[6]
unesp.author.lattes4517484241515548[4]
unesp.author.orcid0000-0002-7455-6867[4]
unesp.author.orcid0000-0002-9231-1994[6]

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