Growth of Candida species on complete dentures: effect of microwave disinfection

dc.contributor.authorSanita, Paula Volpato [UNESP]
dc.contributor.authorVergani, Carlos Eduardo [UNESP]
dc.contributor.authorGiampaolo, Eunice Teresinha [UNESP]
dc.contributor.authorPavarina, Ana Claudia [UNESP]
dc.contributor.authorMachado, Ana Lucia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:46:29Z
dc.date.available2014-05-20T13:46:29Z
dc.date.issued2009-03-01
dc.description.abstractMicrowave disinfection of complete dentures has been recommended to treat denture stomatitis in non-immune compromised patients. Oral candidiasis is a frequent manifestation of HIV infection. The objective of this study is to evaluate the effectiveness of microwave irradiation on the disinfection of complete dentures inoculated with American Type Culture Collection (ATCC) and HIV isolates of five species of Candida. Fifty dentures were made, sterilised and inoculated with the tested microorganisms (C. albicans, C. dubliniensis, C. krusei, C. glabrata and C. tropicalis). After incubation (37 degrees C/48 h), dentures were microwaved (650 W/3 min). Non-irradiated dentures were used as positive controls. Replicate aliquots of suspensions were plated at dilutions 10(-1) to 10(-4) and incubated (37 degrees C/48 h). Colony counts (cfu ml(-1)) were quantified. Dentures were also incubated at 37 degrees C for 7 days. Data were analysed with 2-way anova and Tukey HSD tests (alpha = 0.05). Dentures contaminated with all Candida species showed sterilisation after microwave irradiation. All control dentures showed microbial growth on the plates. The cfu ml(-1) for C. glabrata was higher than those of C. albicans, C. dubliniensis and C. tropicalis whereas the cfu ml(-1) for C. krusei was lower. The cfu ml(-1) for clinical isolates was higher than those of ATCC yeast. Microwave irradiation for 3 min at 650 W resulted in sterilisation of all complete dentures.en
dc.description.affiliationUNESP São Paulo State Univ, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 São Paulo, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 São Paulo, Brazil
dc.format.extent154-160
dc.identifierhttp://dx.doi.org/10.1111/j.1439-0507.2008.01558.x
dc.identifier.citationMycoses. Malden: Wiley-blackwell Publishing, Inc, v. 52, n. 2, p. 154-160, 2009.
dc.identifier.doi10.1111/j.1439-0507.2008.01558.x
dc.identifier.issn0933-7407
dc.identifier.lattes3003130522427820
dc.identifier.lattes5737127334248549
dc.identifier.lattes8867670539105403
dc.identifier.lattes8000248781842587
dc.identifier.orcid0000-0002-7375-4714
dc.identifier.urihttp://hdl.handle.net/11449/16460
dc.identifier.wosWOS:000262901100008
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofMycoses
dc.relation.ispartofjcr2.793
dc.relation.ispartofsjr1,069
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDenture stomatitisen
dc.subjectCandida sppen
dc.subjectdisinfectionen
dc.subjectmicrowavesen
dc.subjectcomplete dentureen
dc.subjectAIDSen
dc.titleGrowth of Candida species on complete dentures: effect of microwave disinfectionen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
unesp.author.lattes3003130522427820[2]
unesp.author.lattes5737127334248549
unesp.author.lattes8867670539105403[4]
unesp.author.lattes8000248781842587[5]
unesp.author.orcid0000-0002-7375-4714[2]
unesp.author.orcid0000-0002-9231-1994[4]
unesp.author.orcid0000-0002-2718-428X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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