Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus

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dc.contributor.authorInui Kishi, Rosangela Naomi
dc.contributor.authorStach-Machado, Dagmar
dc.contributor.authorSingulani, Junya de lacorte [UNESP]
dc.contributor.authorSantos, Claudia Tavares dos [UNESP]
dc.contributor.authorFusco-Almeida, Ana Marisa [UNESP]
dc.contributor.authorCilli, Eduardo Maffud [UNESP]
dc.contributor.authorFreitas-Astua, Juliana
dc.contributor.authorPicchi, Simone Cristina
dc.contributor.authorMachado, Marcos Antonio
dc.contributor.institutionInst Agron
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionFundo Def Citricultura
dc.date.accessioned2018-11-26T17:55:21Z
dc.date.available2018-11-26T17:55:21Z
dc.date.issued2018-09-07
dc.description.abstractAntimicrobial peptides (AMPs) can be found in various organisms, and could be considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X. citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X. citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K-0-W-6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K-0-W-6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X. citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X. citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens.en
dc.description.affiliationInst Agron, Ctr Citricultura Sylvio Moreira, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim Araraquara, Dept Bioquim & Tecnol Quim, Sao Paulo, Brazil
dc.description.affiliationEmbrapa Cassava & Fruits, Cruz Das Almas, BA, Brazil
dc.description.affiliationFundo Def Citricultura, FUNDECITRUS, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim Araraquara, Dept Bioquim & Tecnol Quim, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/11072-4
dc.description.sponsorshipIdFAPESP: 2012/15346-7
dc.description.sponsorshipIdFAPESP: 2008/52691-9
dc.format.extent18
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0203451
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 13, n. 9, 18 p., 2018.
dc.identifier.doi10.1371/journal.pone.0203451
dc.identifier.fileWOS000444093600076.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/164626
dc.identifier.wosWOS:000444093600076
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleEvaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrusen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.lattes3716273524139678[5]
unesp.author.orcid0000-0002-2115-8988[5]

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Artigos - Bioquímica e Tecnologia - IQ