Application of Factorial Design Experiments to the Development of a Disposable Amperometric DNA Biosensor

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dc.contributor.authorUliana, Carolina Venturini [UNESP]
dc.contributor.authorTognolli, Joao Olimpio [UNESP]
dc.contributor.authorYamanaka, Hideko [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:19:09Z
dc.date.available2014-05-20T14:19:09Z
dc.date.issued2011-11-01
dc.description.abstractThe development of an amperometric DNA-based biosensor (genosensor) requires the optimization of many experimental parameters in order to maximize sensitivity. If only one parameter is evaluated per experiment, the resultant genosensor is unlikely to be ideal, because several of the parameters could be interdependent. Herein, chemometric experiments employing full and fractional factorial designs were used to develop biosensor for hepatitis. The results demonstrate that the use of chemometric tools enables optimization of experimental conditions using a smaller number of experiments and with reduced requirements for reagents and samples, which is in line with the principles of green chemistry.en
dc.description.affiliationSão Paulo State Univ UNESP, Inst Chem Araraquara, Dept Analyt Chem, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Inst Chem Araraquara, Dept Analyt Chem, São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 134868/2007-2
dc.description.sponsorshipIdCNPq: 47557/2007-8
dc.format.extent2607-2615
dc.identifierhttp://dx.doi.org/10.1002/elan.201100269
dc.identifier.citationElectroanalysis. Weinheim: Wiley-v C H Verlag Gmbh, v. 23, n. 11, p. 2607-2615, 2011.
dc.identifier.doi10.1002/elan.201100269
dc.identifier.issn1040-0397
dc.identifier.lattes1923726000036625
dc.identifier.urihttp://hdl.handle.net/11449/25769
dc.identifier.wosWOS:000297311500017
dc.language.isoeng
dc.publisherWiley-v C H Verlag Gmbh
dc.relation.ispartofElectroanalysis
dc.relation.ispartofjcr2.851
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectChemometricsen
dc.subjectDNAen
dc.subjectFactorial designen
dc.subjectGenosensoren
dc.subjectHepatitis C virusen
dc.titleApplication of Factorial Design Experiments to the Development of a Disposable Amperometric DNA Biosensoren
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-v C H Verlag Gmbh
unesp.author.lattes1923726000036625
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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Artigos - Química Analítica - IQ