Cryopreservation effects on domestic cat epididymal versus electroejaculated spermatozoa

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dc.contributor.authorTebet, J. M.
dc.contributor.authorMartins, M. I. M.
dc.contributor.authorChirinea, V. H.
dc.contributor.authorSouza, Fabiana Ferreira de [UNESP]
dc.contributor.authorCampagnol, D.
dc.contributor.authorLopes, Maria Denise [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniv Sao Jose
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-02-26T17:26:41Z
dc.date.accessioned2014-05-20T13:41:39Z
dc.date.available2014-02-26T17:26:41Z
dc.date.available2014-05-20T13:41:39Z
dc.date.issued2006-10-01
dc.description.abstractFrozen-thawed epididymal spermatozoa have already been successfully used in artificial insemination in the domestic cat, proving to be a valuable resource for the reproduction of felid species, which are threatened with extinction. The aim of this study was to compare the effects of freezing and thawing on domestic cat semen collected by electroejaculation (EL) and from the epididymides (EP) and vasa deferentia. Ten adult cats were anesthetized, electroejaculated and immediately thereafter, orchiectomized. Epididymal spermatozoa were collected through the compression of caudae epididymidis and vasa deferentia. Spermatozoa were frozen-thawed following a single protocol. Sperm motility, sperm progressive status (0-5), plasma membrane integrity and morphology (light and transmission electron microscope) were assessed on two occasions, immediately after collection and after freezing and thawing. There were no significant differences between the electroejaculated and epididymal fresh or frozen-thawed spermatozoa for any of the variables. However, the incidence of acrosome defects after freezing and thawing increased by 19% based on light microscopy, whereas ultrastructural images revealed acrosome damages in most sperm cells. Since these acrosomal changes are known to affect sperm fertilising capacity, further studies are needed to optimize cryopreservation techniques for epididymal as well as electroejaculated domestic cat spermatozoa. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Dept Reprod Anim & Radiol Vet, Fac Med Vet & Zootecn, BR-18100000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Londrina, Londrina, PR, Brazil
dc.description.affiliationUniv Sao Jose, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationDept Cirugia & Anestesiol Vet, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Reprod Anim & Radiol Vet, Fac Med Vet & Zootecn, BR-18100000 Botucatu, SP, Brazil
dc.format.extent1629-1632
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2006.02.013
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 66, n. 6-7, p. 1629-1632, 2006.
dc.identifier.doi10.1016/j.theriogenology.2006.02.013
dc.identifier.issn0093-691X
dc.identifier.lattes6666129914663018
dc.identifier.urihttp://hdl.handle.net/11449/14434
dc.identifier.wosWOS:000240887300043
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdomestic catpt
dc.subjectcryopreservationpt
dc.subjectsemen freezingpt
dc.subjectspermatozoapt
dc.subjectacrosomept
dc.titleCryopreservation effects on domestic cat epididymal versus electroejaculated spermatozoaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes6666129914663018
unesp.author.orcid0000-0003-4721-1801[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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