Thin-layer chromatography of mycobactins and mycolic acids for the identification of clinical mycobacteria

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dc.contributor.authorLeite, CQF
dc.contributor.authorBarreto, AMW
dc.contributor.authorLeite, SRA
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:21:55Z
dc.date.available2014-05-20T15:21:55Z
dc.date.issued1995-07-01
dc.description.abstractForty seven strains of mycobacteria (35 strains isolated from clinical specimens and 12 reference strains) were analyzed for mycobactin and mycolate production by thin-layer chromatography (TLC). Different growth conditions had little or no effect on the production of individual mycobactins and the reproducibility of mycobactin Rf values. Mycolate profiles of isolated strains were compared with those of reference strains. Clinical isolates belonging to the same species showed the same profiles. The combined evaluation of mycobacterial products by TLC allowed the identification of pathogenic and opportunist cultivable mycobacteria. on routine examination, the analysis of mycobactin and mycolate production constitutes an adequate procedure for the characterization and identification of myobacteria.en
dc.description.affiliationUNESP,FAC CIENCIAS FARMACEUT,DEPT CIENCIAS BIOL,RODOVIA ARARAQUARA JAU KM 1,BR-14801902 ARARAQUARA,SP,BRAZIL
dc.description.affiliationUnespUNESP,FAC CIENCIAS FARMACEUT,DEPT CIENCIAS BIOL,RODOVIA ARARAQUARA JAU KM 1,BR-14801902 ARARAQUARA,SP,BRAZIL
dc.format.extent192-199
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_issues&pid=0001-3714&lng=en&nrm=iso
dc.identifier.citationRevista de Microbiologia. São Paulo: Soc Brasileira Microbiologia, v. 26, n. 3, p. 192-199, 1995.
dc.identifier.issn0001-3714
dc.identifier.lattes2114570774349859
dc.identifier.urihttp://hdl.handle.net/11449/32997
dc.identifier.wosWOS:A1995TL20200007
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofRevista de Microbiologia
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmycobacteriapt
dc.subjectidentificationpt
dc.subjectmycobactinpt
dc.subjectmycolic acidpt
dc.subjectthin layerpt
dc.subjectchromatographypt
dc.titleThin-layer chromatography of mycobactins and mycolic acids for the identification of clinical mycobacteriaen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/rm/paboutj.htm
dcterms.rightsHolderSoc Brasileira Microbiologia
unesp.author.lattes2114570774349859
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Ciências Biológicas - FCFAR