POLYLACTIC/POLYGLYCOLIC ACID COPOLYMER IS A GOOD CARRIER FOR BMP-2 ON BONE REGENERATION?

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dc.contributor.authorVeloso dos Anjos, Alexandre Ladeira
dc.contributor.authorPerez, Roble Casares
dc.contributor.authorBraga, Barbara Maciel
dc.contributor.authorMatsumoto, Mariza Akemi [UNESP]
dc.contributor.authorOkamoto, Roberta [UNESP]
dc.contributor.authorSaraiva, Patricia Pinto
dc.contributor.authorSilva, Andreia Aparecida da
dc.contributor.authorPegoraro, Thiago Amadei
dc.contributor.institutionUniv Sagrado Coracao
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T17:33:02Z
dc.date.available2018-11-26T17:33:02Z
dc.date.issued2017-05-01
dc.description.abstractThe aim of this work was to evaluate the role of polylactic /polyglycolic acid copolymer as carrier for BMP-2 on bone regeneration in rat calvarium. Forty five adult male rats underwent 5-mm critical defects in bone calvaria to be divided into three groups according to the filling materials: Control-blood clot; PLGA-polylactic acid Polyglycolic/copolymer; PLGA + BMP-2 -polylactic acid Polyglycolic/copolymer associated with BMP-2. Sacrifice of animals occurred at 5, 15 and 30 days after surgery The evaluation of new bone formation was obtained by histomorphometry, while OPG and RANKL proteins were observed by immunohistochemistry. Statistical analysis was performed by means of nonparametric tests based on quantitative variables of independent samples. Considering the amount of newly formed bone, significant difference was detected between PGLA (178,2 +/- 137,5 mu m) and the other groups, at day 30. In PLGA + BMP-2 and control groups, the expression of RANKL was prevalent on the OPG in the periods of 15 and 30 days, suggesting a favorable condition for bone reabsorption in these periods. Therefore, immunoexpression of RANKL and OPG and bone formation observed in different groups and periods of analysis showed that the polylactic/polyglycolic acid copolymer does not act as a good carrier for BMP-2.en
dc.description.affiliationUniv Sagrado Coracao, Proreitoria Pesquisa & Posgrad, Bauru, SP, Brazil
dc.description.affiliationUniv Sagrado Coracao, Ctr Ciencias Saude, Bauru, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Ciencias Basicas, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Ciencias Basicas, Aracatuba, SP, Brazil
dc.format.extent815-823
dc.identifier.citationBioscience Journal. Uberlandia: Univ Federal Uberlandia, v. 33, n. 3, p. 815-823, 2017.
dc.identifier.issn1981-3163
dc.identifier.lattes8621510886887389
dc.identifier.urihttp://hdl.handle.net/11449/162837
dc.identifier.wosWOS:000402204400030
dc.language.isoeng
dc.publisherUniv Federal Uberlandia
dc.relation.ispartofBioscience Journal
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBone tissue
dc.subjectOsteoprotegerin
dc.subjectRANKL
dc.subjectImmunohistochemistry
dc.titlePOLYLACTIC/POLYGLYCOLIC ACID COPOLYMER IS A GOOD CARRIER FOR BMP-2 ON BONE REGENERATION?en
dc.typeArtigo
dcterms.rightsHolderUniv Federal Uberlandia
unesp.author.lattes8621510886887389

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