Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle

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dc.contributor.authorGrisolia, A. B. [UNESP]
dc.contributor.authorD'Angelo, G. T. [UNESP]
dc.contributor.authorPorto Neto, L. R. [UNESP]
dc.contributor.authorSiqueira, F.
dc.contributor.authorGarcia, José Fernando [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal da Grande Dourados (UFGD)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2013-09-30T18:25:15Z
dc.date.accessioned2014-05-20T13:42:37Z
dc.date.available2013-09-30T18:25:15Z
dc.date.available2014-05-20T13:42:37Z
dc.date.issued2009-01-01
dc.description.abstractThe myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue and Blond' Aquitaine, and others) show polymorphisms in this gene; these polymorphisms are directly related to the double muscling phenotype. We looked for polymorphisms in the Nellore cattle myostatin gene and compared them with those known for taurine breeds. Seven regions, covering the three exons of this gene, were amplified by polymerase chain reaction and sequenced, including the untranslated region. DNA from 30 adult Nellore animals was collected; DNA sequencing revealed three, seven and four polymorphisms in exons 1, 2 and 3, respectively. We found previously reported polymorphisms, as well as several new ones; for instance, 37 polymorphisms were found in the untranslated region segment, and in introns 1 and 2 there were one and three polymorphisms, respectively. The high degree of allelic heterogeneity in the myostatin gene could be related to its high mutation rate; it also could be the result of a long history of artificial selection for meat production, which has probably favored such modifications and maintained them in cattle populations. These polymorphisms identified in Nellore cattle could be useful for breeding programs.en
dc.description.affiliationUniv Estadual Paulista, Dept Apoio Prod & Saúde Anim, Lab Bioquim & Biol Mol Anim, Aracatuba, SP, Brazil
dc.description.affiliationUFGD, Fac Ciencias Biol & Ambientais, Dourados, MS, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Gado Corte, Campo Grande, MS, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Apoio Prod & Saúde Anim, Lab Bioquim & Biol Mol Anim, Aracatuba, SP, Brazil
dc.description.sponsorshipUNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent822-830
dc.identifierhttp://dx.doi.org/10.4238/vol8-3gmr548
dc.identifier.citationGenetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 8, n. 3, p. 822-830, 2009.
dc.identifier.doi10.4238/vol8-3gmr548
dc.identifier.fileWOS000268634100008.pdf
dc.identifier.issn1676-5680
dc.identifier.lattes9991374083045897
dc.identifier.urihttp://hdl.handle.net/11449/14819
dc.identifier.wosWOS:000268634100008
dc.language.isoeng
dc.publisherFunpec-editora
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectAnimal breedingen
dc.subjectDNA sequencingen
dc.subjectDouble musclingen
dc.subjectMeat productionen
dc.subjectSingle nucleotide polymorphismsen
dc.titleMyostatin (GDF8) single nucleotide polymorphisms in Nellore cattleen
dc.typeArtigo
dcterms.licensehttp://www.geneticsmr.com/node/2
dcterms.rightsHolderFunpec-editora
unesp.author.lattes9991374083045897
unesp.author.orcid0000-0002-3536-8265[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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