HMGB1 Localization during Experimental Periodontitis

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dc.contributor.authorBoas Nogueira, Andressa Vilas [UNESP]
dc.contributor.authorChaves de Souza, Joao Antonio [UNESP]
dc.contributor.authorMolon, Rafael Scaf de [UNESP]
dc.contributor.authorMariano Pereira, Elyne da Silva [UNESP]
dc.contributor.authorAquino, Sabrina Garcia de [UNESP]
dc.contributor.authorGiannobile, William V.
dc.contributor.authorCirelli, Joni Augusto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Michigan
dc.date.accessioned2014-12-03T13:10:46Z
dc.date.available2014-12-03T13:10:46Z
dc.date.issued2014-01-01
dc.description.abstractAim. This study sought to investigate the in vitro expression profile of high mobility group box 1 (HMGB1) in murine periodontal ligament fibroblasts (mPDL) stimulated with LPS or IL-1 beta and in vivo during ligature-or LPS-induced periodontitis in rats. Material and Methods. For the in vivo study, 36 rats were divided into experimental and control groups, and biopsies were harvested at 7-30 d following disease induction. Bone loss and inflammation were evaluated. HMGB1 expression was assessed by immunohistochemistry, qPCR, and Western blot. Results. Significant increases in mPDL HMGB1 mRNA occurred at 4, 8, and 12 h with protein expression elevated by 24 h. HMGB1 mRNA expression in gingival tissues was significantly increased at 15 d in the LPS-PD model and at 7 and 15 d in the ligature model. Immunohistochemical staining revealed a significant increase in the number of HMGB1-positive cells during the experimental periods. Conclusion. The results show that PDL cells produce HMGB1, which is increased and secreted extracellularly after inflammatory stimuli. In conclusion, this study demonstrates that HMGB1 may be associated with the onset and progression of periodontitis, suggesting that further studies should investigate the potential role of HMGB1 on periodontal tissue destruction.en
dc.description.affiliationUniv Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniv Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 10/05957-3
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1155/2014/816320
dc.identifier.citationMediators Of Inflammation. New York: Hindawi Publishing Corporation, 10 p., 2014.
dc.identifier.doi10.1155/2014/816320
dc.identifier.fileWOS000332291900001.pdf
dc.identifier.issn0962-9351
dc.identifier.lattes2628593693450121
dc.identifier.urihttp://hdl.handle.net/11449/112503
dc.identifier.wosWOS:000332291900001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofMediators of Inflammation
dc.relation.ispartofjcr3.549
dc.relation.ispartofsjr1,370
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleHMGB1 Localization during Experimental Periodontitisen
dc.typeArtigo
dcterms.rightsHolderHindawi Publishing Corporation
unesp.author.lattes2628593693450121
unesp.author.orcid0000-0003-1110-6233[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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