Effect of manganese supplementation on the membrane integrity and the mitochondrial potential of the sperm of grazing Nelore bulls

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dc.contributor.authorReis, L. S. L. S. [UNESP]
dc.contributor.authorRamos, Alcides Amorim [UNESP]
dc.contributor.authorCamargos, A. S. [UNESP]
dc.contributor.authorOba, Eunice [UNESP]
dc.contributor.institutionUniv Santo Amaro
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:53:16Z
dc.date.available2015-03-18T15:53:16Z
dc.date.issued2014-11-10
dc.description.abstractThe effect of dietary manganese (Mn2+) supplementation on the reproductive performance of Nelore bulls was evaluated by assessment of sperm membrane integrity. Sixty Nelore bulls (Bos taurus indicus) aged 18-20 mo were randomly divided into four groups (n = 15) receiving dietary Mn2+ supplementation at 540, 1300, 3800 and 6300 mg/kg (treatments T-c, T-1300, T-3800 and T-6300, respectively). The diets were changed for the groups every 70 d. Semen samples were obtained 15 and 56 d after the diet change, which corresponded to the period of adjustment to the new diet and the time required for a complete spermatogenesis cycle, respectively. Sperm integrity was assessed by detection of: intact (IMe) or damaged (DMe) membranes, intact (IA) or damaged (DA) acrosomes, and high (HM) or low (LM) mitochondrial membrane potentials. Only bulls from the Tc treatment showed a significant increase in the production of intact sperm [IMe/IA/LM] and decrease in the production of sperm with damaged acrosome [IMe/DA/LM] or completely damaged sperm IDMe/DA/LM] (P < 0.05). The Mn2+ concentrations in the semen were positively correlated with the incidence of sperm with IMe, DA, and LM and negatively correlated with number of sperm with DMe, IA, and LM. Therefore, dietary Mn2+ supplementation for Nelore bulls must be limited to 540 mg of Mn2+/kg given that higher doses are detrimental to the integrity of the plasma and acrosomal sperm membranes. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Santo Amaro, Dept Anim Nutr & Food Sci, Sao Paulo, Brazil
dc.description.affiliationSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Prod, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Prod, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipTORTUGA Companhia Zootecnica Agraria
dc.format.extent1-6
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2014.06.033
dc.identifier.citationAnimal Reproduction Science. Amsterdam: Elsevier Science Bv, v. 150, n. 1-2, p. 1-6, 2014.
dc.identifier.doi10.1016/j.anireprosci.2014.06.033
dc.identifier.issn0378-4320
dc.identifier.lattes9224380586557664
dc.identifier.urihttp://hdl.handle.net/11449/116408
dc.identifier.wosWOS:000345180000001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAnimal Reproduction Science
dc.relation.ispartofjcr1.647
dc.relation.ispartofsjr0,704
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectManganeseen
dc.subjectSpermatozoaen
dc.subjectIntegrity of sperm membrane and acrosomeen
dc.subjectCattleen
dc.subjectMineral salten
dc.titleEffect of manganese supplementation on the membrane integrity and the mitochondrial potential of the sperm of grazing Nelore bullsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes9224380586557664
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ
Artigos - Produção Animal - FMVZ