Apocynin reduces blood pressure and restores the proper function of vascular endothelium in SHR

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dc.contributor.authorPerassa, Ligia A. [UNESP]
dc.contributor.authorGraton, Murilo E. [UNESP]
dc.contributor.authorPotje, Simone R. [UNESP]
dc.contributor.authorTroiano, Jéssica A. [UNESP]
dc.contributor.authorLima, Mariana S. [UNESP]
dc.contributor.authorVale, Gabriel T.
dc.contributor.authorPereira, Ariana A.F. [UNESP]
dc.contributor.authorNakamune, Ana Cláudia de Melo Stevanato [UNESP]
dc.contributor.authorSumida, Doris H. [UNESP]
dc.contributor.authorTirapelli, Carlos R.
dc.contributor.authorBendhack, Lusiane M.
dc.contributor.authorAntoniali, Cristina [UNESP]
dc.contributor.institutionSBFis
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:08:15Z
dc.date.available2018-12-11T17:08:15Z
dc.date.issued2016-12-01
dc.description.abstractThis study has evaluated how the vascular endothelium of hypertensive rats chronically treated with apocynin affects acetylcholine (ACh), sodium nitroprusside (SNP), and phenylephrine (PE) action on the nitric oxide (NO) signal transduction pathway in endothelial (EC) and vascular smooth muscle cells. Treatment with apocynin significantly reduced the mean arterial pressure in spontaneously hypertensive rats (SHR). In addition, apocynin improved the impaired ACh hypotensive effect on SHR. Although systemic oxidative stress was high in SHR, SHR treated with apocynin and normotensive rats presented similar systemic oxidative stress levels. Endothelium significantly blunted PE contractions in intact aortas of treated SHR. The ACh effect was impaired in resistance arteries and aortas of SHR, but this same effect was improved in treated SHR. The SNP potency was higher in intact resistance arteries of treated SHR than in intact resistance arteries of untreated SHR. NO and calcium concentrations increased, whereas reactive oxygen species levels decreased in EC of treated SHR. Aortas of untreated and treated SHR did not differ in terms of sGC alpha or beta units expression. Aorta of treated SHR expressed higher eNOS levels as compared to aorta of untreated SHR. The study groups did not differ with respect to NOX1, NOXO1, or NOX4 expression. However, treatment with apocynin normalized overexpression of NOX2 and its subunit p47phox in aortas of SHR. Based on all the results presented in this study, we suggest apocynin increases NO biovailability by different mechanisms, restoring the proper function of vascular endothelium in SHR.en
dc.description.affiliationMulticenter Graduate Program in Physiological Sciences SBFis
dc.description.affiliationDepartment of Basic Sciences School of Dentistry of Araçatuba UNESP – Univ Estadual Paulista
dc.description.affiliationDepartment of Psychiatry Nursing and Human Sciences College of Nursing of Ribeirão Preto USP - University of São Paulo
dc.description.affiliationDepartment of Physics and Chemistry Faculty of Pharmaceutical Sciences of Ribeirão Preto USP - University of São Paulo
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry of Araçatuba UNESP – Univ Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/04619-0
dc.description.sponsorshipIdFAPESP: 2011/19859-6
dc.description.sponsorshipIdFAPESP: 2011/20998-0
dc.description.sponsorshipIdFAPESP: 2012/01733-9
dc.format.extent38-48
dc.identifierhttp://dx.doi.org/10.1016/j.vph.2016.06.005
dc.identifier.citationVascular Pharmacology, v. 87, p. 38-48.
dc.identifier.doi10.1016/j.vph.2016.06.005
dc.identifier.file2-s2.0-85003828314.pdf
dc.identifier.issn1879-3649
dc.identifier.issn1537-1891
dc.identifier.scopus2-s2.0-85003828314
dc.identifier.urihttp://hdl.handle.net/11449/173898
dc.language.isoeng
dc.relation.ispartofVascular Pharmacology
dc.relation.ispartofsjr1,352
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectApocynin
dc.subjectEndothelial dysfunction
dc.subjecteNOS
dc.subjectReactive oxygen species
dc.subjectSHR
dc.titleApocynin reduces blood pressure and restores the proper function of vascular endothelium in SHRen
dc.typeArtigo
unesp.author.lattes8110154498443749[8]
unesp.author.orcid0000-0001-5098-8406[8]

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