Fabrication of microenvironments with different geometrical features for cell growth studies

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dc.contributor.authorAvila, O. I.
dc.contributor.authorOtuka, A. J. G.
dc.contributor.authorTribuzi, V.
dc.contributor.authorFreitas, Laura Marise de [UNESP]
dc.contributor.authorSerafim, Rodolfo B. [UNESP]
dc.contributor.authorMoraes, M. H.
dc.contributor.authorEspreafico, E. M.
dc.contributor.authorValente, Valeria [UNESP]
dc.contributor.authorFontana, Carla Raquel [UNESP]
dc.contributor.authorMendonca, C. R.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:56:15Z
dc.date.available2015-03-18T15:56:15Z
dc.date.issued2014-11-01
dc.description.abstractStudies of cell development in artificial microenvironments can contribute to understanding a series of physiological mechanisms that might be influenced by geometrical features of the microenvironment itself. In this work we applied two-photon polymerization to fabricate three-dimensional microenvironments composed of a matrix arrangement of microstructures (circular and square cross-sections pillars) with distinct spacing. Such microenvironments were used to evaluate the growth of Michigan Cancer Foundation-7 (MCF-7) cells that are commonly used as a model system to investigate fundamental aspects of the tumor biology. Our results reveal that the cell density decreases as the distance between structures in the environment is increased. Additionally, cell growth shows slightly better results for the microenvironments composed of circular cross-section structures.en
dc.description.affiliationUniv Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Fac Ciencias Farmaceut, BR-14801960 Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationNAP USP, CISBi Ctr Integrat Syst Biol, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Fac Ciencias Farmaceut, BR-14801960 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 11/12399-0
dc.description.sponsorshipIdFAPESP: 12/21807-7
dc.format.extent248-251
dc.identifierhttp://dx.doi.org/10.2961/jlmn.2014.03.0013
dc.identifier.citationJournal Of Laser Micro Nanoengineering. Osaka: Japan Laser Processing Soc, v. 9, n. 3, p. 248-251, 2014.
dc.identifier.doi10.2961/jlmn.2014.03.0013
dc.identifier.issn1880-0688
dc.identifier.lattes5050653153437898
dc.identifier.orcid0000-0002-3942-7744
dc.identifier.urihttp://hdl.handle.net/11449/117475
dc.identifier.wosWOS:000346184500013
dc.language.isoeng
dc.publisherJapan Laser Processing Soc
dc.relation.ispartofJournal Of Laser Micro Nanoengineering
dc.relation.ispartofjcr0.789
dc.relation.ispartofsjr0,344
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectTwo-photon absortionen
dc.subjectmicrofabricationen
dc.subjectphotopolimerizationen
dc.subjectMichigan Cancer Foundation-7en
dc.subjectGreen Fluorescent Proteinen
dc.titleFabrication of microenvironments with different geometrical features for cell growth studiesen
dc.typeArtigo
dcterms.rightsHolderJapan Laser Processing Soc
unesp.author.lattes5050653153437898
unesp.author.lattes5168319315634298[10]
unesp.author.orcid0000-0002-3942-7744[8]
unesp.author.orcid0000-0002-9135-3690[10]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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