Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients

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dc.contributor.authorMoro Lima Dos Santos, Ana Cláudia [UNESP]
dc.contributor.authorMonteiro, Aydir Cecília Marinho [UNESP]
dc.contributor.authorBarbosa, Thaís Alves [UNESP]
dc.contributor.authorRiboli, Danilo Flávio Moraes [UNESP]
dc.contributor.authorCamargo, Carlos Henrique
dc.contributor.authorFerreira, Adriano Martison [UNESP]
dc.contributor.authorMondelli, Alessandro Lia [UNESP]
dc.contributor.authorMontelli, Augusto Cezar [UNESP]
dc.contributor.authorHernandes, Rodrigo Tavanelli [UNESP]
dc.contributor.authorRibeiro de Souza da Cunha, Maria de Lourdes [UNESP]
dc.contributor.authorBarretti, Pasqual [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto Adolfo Lutz
dc.date.accessioned2022-04-29T08:39:30Z
dc.date.available2022-04-29T08:39:30Z
dc.date.issued2022-02-01
dc.description.abstract(1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome.en
dc.description.affiliationDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
dc.description.affiliationCentro de Bacteriologia Instituto Adolfo Lutz, SP
dc.description.affiliationHospital das Clínicas da Faculdade de Medicina de Botucatu, SP
dc.description.affiliationDepartamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
dc.description.affiliationUnespDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
dc.description.affiliationUnespHospital das Clínicas da Faculdade de Medicina de Botucatu, SP
dc.description.affiliationUnespDepartamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/21379-8
dc.identifierhttp://dx.doi.org/10.3390/pathogens11020218
dc.identifier.citationPathogens, v. 11, n. 2, 2022.
dc.identifier.doi10.3390/pathogens11020218
dc.identifier.issn2076-0817
dc.identifier.scopus2-s2.0-85124426014
dc.identifier.urihttp://hdl.handle.net/11449/230370
dc.language.isoeng
dc.relation.ispartofPathogens
dc.sourceScopus
dc.subjectAcinetobacter
dc.subjectAntimicrobial resistance
dc.subjectBiofilm
dc.subjectClonal profile
dc.subjectNonfermenting Gram-negative bacilli
dc.subjectPeritoneal dialysis
dc.subjectPeritonitis
dc.subjectPseudomonas
dc.subjectVirulence factors
dc.titlePhenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patientsen
dc.typeArtigo

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