Effect of soybean oil and Tween 80 on the production of botryosphaeran by Botryosphaeria rhodina MAMB-05

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Silva, Cassiano C.
Dekker, Robert F. H.
Silva, Rui Sergio S. F.
da Silva, Maria de Lourdes Corradi
Barbosa, Aneli M.

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Elsevier B.V.


The addition of soybean oil and Tween 80 was evaluated with the objective of increasing the production of botryosphaeran, an exopolysaccharide (EPS) of the (1 -> 3 ;1 -> 6)-beta-D-glucan type produced by the fungus Botowsphaeria rhodina MAMB-05. Factorial design and analysis by response surface methodology was developed to select the main factors that would affect and enhance EPS production. The optimized culture conditions were: 40g l(-1) glucose with 10ml l(-1) soybean oil, and 4.5 ml l(-1) Tween 80, during 72h cultivation at 28 degrees C (180 rpm) and initial pH 5.7. The predicted result for botryosphaeran production was 8.22 +/- 1.36 g l(-1), and compared with the experimental value of 7.74 +/- 0.13 g l(-1) . Partial characterization of the botryosphaeran produced under the optimized conditions showed one type of polysaccharide with P-glycosidic linkages containing glucose as monosaccharide. (c) 2007 Elsevier Ltd. All rights reserved.



botryosphaeran, Botryosphaeria rhodina MAMB-05, exopolysaccharide, soybean oil, tween 80, response surface methodology

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Process Biochemistry. Oxford: Elsevier B.V., v. 42, n. 8, p. 1254-1258, 2007.