Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin B and itraconazole

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dc.contributor.authorTakahagi-Nakaira, E. [UNESP]
dc.contributor.authorSugizaki, M. F. [UNESP]
dc.contributor.authorPeracoli, M. T. S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:56Z
dc.date.available2014-05-20T13:50:56Z
dc.date.issued2009-01-01
dc.description.abstractIn vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35 degrees C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 mu g/mL and 0.002 mu g/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 mu g/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 mu g/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs.en
dc.description.affiliationUNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 02/12462-4
dc.description.sponsorshipIdFAPESP: 03/12743-0
dc.format.extent718-731
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992009000400010
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 15, n. 4, p. 718-731, 2009.
dc.identifier.fileS1678-91992009000400010.pdf
dc.identifier.issn1678-9199
dc.identifier.scieloS1678-91992009000400010
dc.identifier.urihttp://hdl.handle.net/11449/18175
dc.identifier.wosWOS:000272488500009
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectamphotericin Ben
dc.subjectitraconazoleen
dc.subjectP. brasiliensisen
dc.subjectantifungal susceptibilityen
dc.titleMicrodilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin B and itraconazoleen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB