Granulocyte macrophage colony-stimulating factor enhances the modulatory effect of cytokines on monocyte-derived multinucleated giant cell formation and fungicidal activity against Paracoccidioides brasiliensis

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dc.contributor.authorNascimento, Magda Paula Pereira do [UNESP]
dc.contributor.authorBannwart, Camila Ferreira [UNESP]
dc.contributor.authorNakaira-Takahagi, Erika [UNESP]
dc.contributor.authorPeraçoli, Maria Terezinha Serrão [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:34:22Z
dc.date.available2014-05-20T13:34:22Z
dc.date.issued2011-09-01
dc.description.abstractMultinucleated giant cells (MGC) are cells present in characteristic granulomatous inflammation induced by intracellular infectious agents or foreign materials. The present study evaluated the modulatory effect of granulocyte macrophage colony-stimulating factor (GM-CSF) in association with other cytokines such as interferon-gamma (IFN-γ), tumour necrosis factor-alpha, interleukin (IL)-10 or transforming growth factor beta (TGF-β1) on the formation of MGC from human peripheral blood monocytes stimulated with Paracoccidioides brasiliensis antigen (PbAg). The generation of MGC was determined by fusion index (FI) and the fungicidal activity of these cells was evaluated after 4 h of MGC co-cultured with viable yeast cells of P. brasiliensis strain 18 (Pb18). The results showed that monocytes incubated with PbAg and GM-CSF plus IFN-γ had a significantly higher FI than in all the other cultures, while the addition of IL-10 or TGF-β1 had a suppressive effect on MGC generation. Monocytes incubated with both pro and anti-inflammatory cytokines had a higher induction of foreign body-type MGC rather than Langhans-type MGC. MGC stimulated with PbAg and GM-CSF in association with the other cytokines had increased fungicidal activity and the presence of GM-CSF also partially inhibited the suppressive effects of IL-10 and TGF-β1. Together, these results suggest that GM-CSF is a positive modulator of PbAg-stimulated MGC generation and on the fungicidal activity against Pb18.en
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências Faculdade de Medicina
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e Imunologia
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências Faculdade de Medicina
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e Imunologia
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 03/13743-0
dc.format.extent735-741
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600014
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 6, p. 735-741, 2011.
dc.identifier.doi10.1590/S0074-02762011000600014
dc.identifier.fileS0074-02762011000600014.pdf
dc.identifier.issn0074-0276
dc.identifier.lattes6486557387397806
dc.identifier.scieloS0074-02762011000600014
dc.identifier.urihttp://hdl.handle.net/11449/11773
dc.identifier.wosWOS:000296022400014
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectParacoccidioides brasiliensisen
dc.subjectcytokinesen
dc.subjectmultinucleated giant cellsen
dc.subjectfungicidal activityen
dc.titleGranulocyte macrophage colony-stimulating factor enhances the modulatory effect of cytokines on monocyte-derived multinucleated giant cell formation and fungicidal activity against Paracoccidioides brasiliensisen
dc.typeArtigo
unesp.advisor.lattes6486557387397806
unesp.author.orcid0000-0002-0936-9512[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB