Experimental glaucoma model (ischemia and reperfusion): histology, morphometry, protein and gene espression of apoptosis pathway

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dc.contributor.advisorAmorim, Renée Laufer [UNESP]
dc.contributor.advisorLaus, José Luiz [UNESP]
dc.contributor.authorZanoni, Diogo Sousa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-10T14:23:03Z
dc.date.available2015-12-10T14:23:03Z
dc.date.issued2015-05-28
dc.description.abstractPurpose: The aims of this study were to better understand the mechanism of cell death by apoptosis in a glaucoma model (ischemia / reperfusion) and evaluate the role of apoptosis in this model and if treatment with Sildenafil helps prevent apoptosis. Methods: 36 rats, from 4 to 6 months, males, Lewis and weighing ± 350g were divided in 5 groups: control group (6 animals) and for groups with ischemia / reperfusion (7 and 21 days), two groups consisting of ten animals treated with sildenafil and two groups of Five animals treated with placebo. Paracentesis of the anterior chamber with needle 30G coupled to saline (0.9%) was made and maintained for 60 minutes. Intraocular pressure was measured by rebound tonometer (Tonovet®). There was histological, morphometric by hematoxylin and eosin and, immunohistochemical staining and qRT-PCR analysis by Caspase-7, Caspase-6, Caspase-9, Tnf-r2, Fas-l, Bcl-2 and Bax. For statistic analysis we used ANOVA and t-test for morphometric analysis and, for immunohistochemistry and qRT-PCR, Fisher exact test was employed with a statistical significance level of p <0.05 Results: Histology and morphometric analysis, proved more changes in the untreated group compared to the treatment and control group. Analysis of immunohistochemistry and qRT-PCR observed the more significant expression in untreated eyes. Conclusion: Sildenafil apperead to be protective to ganglion cell apoptosis. Cell survival was evident in histology and morphometry. For immunohistochemistry and RT-PCR was observed protective effect in the apoptosis pathways with similar or below expression compared to the controlen
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent46 f.
dc.identifier.aleph000852681
dc.identifier.capes33004064022P3
dc.identifier.citationZANONI, Diogo Sousa. Experimental glaucoma model (ischemia and reperfusion): histology, morphometry, protein and gene espression of apoptosis pathway. 2015. 46 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2015.
dc.identifier.filehttp://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/06-11-2015/000852681.pdf
dc.identifier.lattes9795829022108105
dc.identifier.urihttp://hdl.handle.net/11449/132013
dc.language.isopor
dc.publisherUniversidade Estadual Paulista (Unesp)
dc.rights.accessRightsAcesso aberto
dc.sourceAleph
dc.subjectApoptosept
dc.subjectGlaucomapt
dc.subjectIsquemiapt
dc.subjectReperfusão (Fisiologia)pt
dc.subjectRetina - Doençaspt
dc.subjectRetina - Diseasespt
dc.titleExperimental glaucoma model (ischemia and reperfusion): histology, morphometry, protein and gene espression of apoptosis pathwaypt
dc.typeDissertação de mestrado
unesp.author.lattes9795829022108105
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.graduateProgramMedicina Veterinária - FMVZpt
unesp.knowledgeAreaAnatomia e patologia animalpt
unesp.researchAreaPatologia animalpt

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Dissertações - Medicina Veterinária - FMVZ